Regulation of cyclin D1, DNA topoisomerase I, and proliferating cell nuclear antigen promoters during the cell cycle.

H. H. Lee, W. H. Chiang, S. H. Chiang, Y. C. Liu, J. Hwang, S. Y. Ng

研究成果: 雜誌貢獻文章

40 引文 (Scopus)

摘要

Cyclin D1, DNA topoisomerase I, and proliferating cell nuclear antigen (PCNA) are three important cell cycle regulatory proteins. Recently, their promoters have been isolated, thus facilitating molecular analysis of transcriptional control mechanisms of these genes. Transcription of these three promoters in stable K562 transfectants during different cell cycle phases was analyzed after cell cycle synchronization. About 1 kb of 5' flanking region from either cyclin D1 or DNA topoisomerase I gene is sufficient to confer G1- or S-phase-specific transcription activity to chloramphenicol acetyltransferase (CAT) reporter genes, respectively. In contrast, 2.8 kb of 5' flanking sequences from the PCNA gene led to constitutive transcription, but the inclusion of a segment of the PCNA gene first intron, which contains evolutionarily conserved sequences, could enhance transcription in G1/S-enriched nuclei. This PCNA intron region contains a binding site recognized by the transcription factor E2F. To test whether this site is functional, we cotransfected PCNA-CAT genes with E2F-1 and DP-1 expression plasmids. Expression of the E2F-1/DP-1 heterodimer activated the CAT gene with the PCNA intron. Therefore, this intron region, involved in transcriptional activation at the cell cycle G1/S boundary, is also E2F inducible.

原文英語
頁(從 - 到)95-109
頁數15
期刊Gene Expression
4
發行號3
出版狀態已發佈 - 1995
對外發佈Yes

指紋

Type I DNA Topoisomerase
Cyclin D1
Proliferating Cell Nuclear Antigen
Cell Cycle
Introns
Chloramphenicol O-Acetyltransferase
Genes
5' Flanking Region
E2F Transcription Factors
Cell Cycle Proteins
Conserved Sequence
S Phase
Reporter Genes
Transcriptional Activation
Plasmids
Binding Sites

ASJC Scopus subject areas

  • Genetics

引用此文

Lee, H. H., Chiang, W. H., Chiang, S. H., Liu, Y. C., Hwang, J., & Ng, S. Y. (1995). Regulation of cyclin D1, DNA topoisomerase I, and proliferating cell nuclear antigen promoters during the cell cycle. Gene Expression, 4(3), 95-109.

Regulation of cyclin D1, DNA topoisomerase I, and proliferating cell nuclear antigen promoters during the cell cycle. / Lee, H. H.; Chiang, W. H.; Chiang, S. H.; Liu, Y. C.; Hwang, J.; Ng, S. Y.

於: Gene Expression, 卷 4, 編號 3, 1995, p. 95-109.

研究成果: 雜誌貢獻文章

Lee, HH, Chiang, WH, Chiang, SH, Liu, YC, Hwang, J & Ng, SY 1995, 'Regulation of cyclin D1, DNA topoisomerase I, and proliferating cell nuclear antigen promoters during the cell cycle.', Gene Expression, 卷 4, 編號 3, 頁 95-109.
Lee, H. H. ; Chiang, W. H. ; Chiang, S. H. ; Liu, Y. C. ; Hwang, J. ; Ng, S. Y. / Regulation of cyclin D1, DNA topoisomerase I, and proliferating cell nuclear antigen promoters during the cell cycle. 於: Gene Expression. 1995 ; 卷 4, 編號 3. 頁 95-109.
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