Reduction of indocyanine green-associated photosensitizing toxicity in retinal pigment epithelium by sodium elimination

Jau D. Ho, Hung Chiao Chen, San N. Chen, Jui-Fang Tsai

研究成果: 雜誌貢獻文章

25 引文 (Scopus)

摘要

Objective: To determine if eliminating sodium affects indocyanine green (ICG) photosensitizing toxicity and uptake in cultured human retinal pigment epithelial (RPE) cells. Methods: Cultured human RPE cells were exposed to ICG (2.5 mg/mL) in balanced salt solution and sodium-free balanced salt solution for 2 minutes. Afterwards, ICG was removed, and the cells were irradiated with a light beam (4 × 104 lux) for 40 minutes. Toxicity was monitored using light microscopy, calcein AM-ethidium homodimer 1 staining, trypan blue exclusion test, and 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl) -2H-tetrazolium viability assay. Indocyanine green uptake was measured by optical absorption at 790 nm. Results: Photoreactive changes occurred in RPE cells exposed td ICG and light. These changes included cell shrinkage, cell death, pyknotic nuclei, reduced viability, and reduced mitochondrial dehydrogenase activity. These changes were less severe when ICG was dissolved in sodium-free balanced salt solution. In addition, ICG uptake was reduced when the solvent was sodium-free balanced salt solution. Conclusion: Indocyanine green and intense light exposure in RPE cells caused photosensitizing toxicity that was reduced when sodium in the solvent was eliminated and replaced with other cations. Clinical Relevance: Eliminating sodium from the solvent reduced ICG uptake into RPE and its associated photosensitizing toxicity. This reconstitution method of ICG may be helpful for safer intravitreal ICG use in macular hole surgery.
原文英語
頁(從 - 到)871-878
頁數8
期刊Archives of Ophthalmology
122
發行號6
DOIs
出版狀態已發佈 - 六月 2004

指紋

Indocyanine Green
Retinal Pigment Epithelium
Sodium
Retinal Pigments
Salts
Epithelial Cells
Light
Retinal Perforations
Trypan Blue
Cations
Microscopy
Oxidoreductases
Cell Death
Staining and Labeling

ASJC Scopus subject areas

  • Ophthalmology

引用此文

Reduction of indocyanine green-associated photosensitizing toxicity in retinal pigment epithelium by sodium elimination. / Ho, Jau D.; Chen, Hung Chiao; Chen, San N.; Tsai, Jui-Fang.

於: Archives of Ophthalmology, 卷 122, 編號 6, 06.2004, p. 871-878.

研究成果: 雜誌貢獻文章

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abstract = "Objective: To determine if eliminating sodium affects indocyanine green (ICG) photosensitizing toxicity and uptake in cultured human retinal pigment epithelial (RPE) cells. Methods: Cultured human RPE cells were exposed to ICG (2.5 mg/mL) in balanced salt solution and sodium-free balanced salt solution for 2 minutes. Afterwards, ICG was removed, and the cells were irradiated with a light beam (4 × 104 lux) for 40 minutes. Toxicity was monitored using light microscopy, calcein AM-ethidium homodimer 1 staining, trypan blue exclusion test, and 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl) -2H-tetrazolium viability assay. Indocyanine green uptake was measured by optical absorption at 790 nm. Results: Photoreactive changes occurred in RPE cells exposed td ICG and light. These changes included cell shrinkage, cell death, pyknotic nuclei, reduced viability, and reduced mitochondrial dehydrogenase activity. These changes were less severe when ICG was dissolved in sodium-free balanced salt solution. In addition, ICG uptake was reduced when the solvent was sodium-free balanced salt solution. Conclusion: Indocyanine green and intense light exposure in RPE cells caused photosensitizing toxicity that was reduced when sodium in the solvent was eliminated and replaced with other cations. Clinical Relevance: Eliminating sodium from the solvent reduced ICG uptake into RPE and its associated photosensitizing toxicity. This reconstitution method of ICG may be helpful for safer intravitreal ICG use in macular hole surgery.",
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N2 - Objective: To determine if eliminating sodium affects indocyanine green (ICG) photosensitizing toxicity and uptake in cultured human retinal pigment epithelial (RPE) cells. Methods: Cultured human RPE cells were exposed to ICG (2.5 mg/mL) in balanced salt solution and sodium-free balanced salt solution for 2 minutes. Afterwards, ICG was removed, and the cells were irradiated with a light beam (4 × 104 lux) for 40 minutes. Toxicity was monitored using light microscopy, calcein AM-ethidium homodimer 1 staining, trypan blue exclusion test, and 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl) -2H-tetrazolium viability assay. Indocyanine green uptake was measured by optical absorption at 790 nm. Results: Photoreactive changes occurred in RPE cells exposed td ICG and light. These changes included cell shrinkage, cell death, pyknotic nuclei, reduced viability, and reduced mitochondrial dehydrogenase activity. These changes were less severe when ICG was dissolved in sodium-free balanced salt solution. In addition, ICG uptake was reduced when the solvent was sodium-free balanced salt solution. Conclusion: Indocyanine green and intense light exposure in RPE cells caused photosensitizing toxicity that was reduced when sodium in the solvent was eliminated and replaced with other cations. Clinical Relevance: Eliminating sodium from the solvent reduced ICG uptake into RPE and its associated photosensitizing toxicity. This reconstitution method of ICG may be helpful for safer intravitreal ICG use in macular hole surgery.

AB - Objective: To determine if eliminating sodium affects indocyanine green (ICG) photosensitizing toxicity and uptake in cultured human retinal pigment epithelial (RPE) cells. Methods: Cultured human RPE cells were exposed to ICG (2.5 mg/mL) in balanced salt solution and sodium-free balanced salt solution for 2 minutes. Afterwards, ICG was removed, and the cells were irradiated with a light beam (4 × 104 lux) for 40 minutes. Toxicity was monitored using light microscopy, calcein AM-ethidium homodimer 1 staining, trypan blue exclusion test, and 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl) -2H-tetrazolium viability assay. Indocyanine green uptake was measured by optical absorption at 790 nm. Results: Photoreactive changes occurred in RPE cells exposed td ICG and light. These changes included cell shrinkage, cell death, pyknotic nuclei, reduced viability, and reduced mitochondrial dehydrogenase activity. These changes were less severe when ICG was dissolved in sodium-free balanced salt solution. In addition, ICG uptake was reduced when the solvent was sodium-free balanced salt solution. Conclusion: Indocyanine green and intense light exposure in RPE cells caused photosensitizing toxicity that was reduced when sodium in the solvent was eliminated and replaced with other cations. Clinical Relevance: Eliminating sodium from the solvent reduced ICG uptake into RPE and its associated photosensitizing toxicity. This reconstitution method of ICG may be helpful for safer intravitreal ICG use in macular hole surgery.

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