Reduced expression of circadian clock genes in male alcoholic patients

Ming Chyi Huang, Chia Wei Ho, Chun Hsin Chen, Shing Cheng Liu, Chiao Chicy Chen, Sy Jye Leu

研究成果: 雜誌貢獻文章

47 引文 (Scopus)

摘要

Background: There are clear interactions between chronic alcohol consumption and circadian rhythmicity that is regulated by several circadian clock genes. The altered expressions of these genes have been mainly described in animals. The mammalian master clock in the suprachiasmatic nuclei orchestrates the biological rhythms in peripheral tissues. As peripheral blood mononuclear cells (PBMCs) are the most accessible tissue clinically, we assessed the mRNA levels of these genes in patients with alcohol dependence (AD) undergoing alcohol-withdrawal (AW) treatment.Methods: Twenty-two male patients fulfilled the DSM-IV diagnostic criteria of AD, and 12 comparison healthy control subjects were recruited. The patients with AD were further divided by the presence of delirium tremens (DTs), the most severe form of AW syndrome, into DT group and non-DT group. All the participants received blood withdrawal at 9 am, while the patients with AD had blood collection twice: on the next morning of admission (baseline) and on the seventh day. PBMCs were isolated from whole blood, and the mRNA expression profiles of hClock1, hBmal1, hPer1, hPer2, hCry1, and hCry2 were determined by quantitative real-time PCR.Results: The baseline mRNA levels of the target circadian clock genes were markedly lower in patients with AD than in control subjects. After 1 week of alcohol detoxification, there were very limited restorations of discrete circadian gene expressions. DT group did not differ in the expression patterns of circadian clock genes from non-DT group.Conclusions: This is the first study demonstrating the overall lowering of circadian clock genes among patients with AD. The expression pattern is comparable between patients with and without DTs. Although preliminary with data at only one single time point, the observation of strikingly reduced mRNA levels supports the association between circadian clock gene dysregulation and chronic alcohol intake.

原文英語
頁(從 - 到)1899-1904
頁數6
期刊Alcoholism: Clinical and Experimental Research
34
發行號11
DOIs
出版狀態已發佈 - 十一月 2010

指紋

Circadian Clocks
Alcoholics
Clocks
Alcohol Withdrawal Delirium
Alcoholism
Genes
Alcohols
Blood
Messenger RNA
Periodicity
Blood Cells
Gene Expression
Suprachiasmatic Nucleus
Diagnostic and Statistical Manual of Mental Disorders
Alcohol Drinking
Tissue
Real-Time Polymerase Chain Reaction
Healthy Volunteers
Detoxification
Observation

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Psychiatry and Mental health
  • Toxicology

引用此文

Reduced expression of circadian clock genes in male alcoholic patients. / Huang, Ming Chyi; Ho, Chia Wei; Chen, Chun Hsin; Liu, Shing Cheng; Chen, Chiao Chicy; Leu, Sy Jye.

於: Alcoholism: Clinical and Experimental Research, 卷 34, 編號 11, 11.2010, p. 1899-1904.

研究成果: 雜誌貢獻文章

Huang, Ming Chyi ; Ho, Chia Wei ; Chen, Chun Hsin ; Liu, Shing Cheng ; Chen, Chiao Chicy ; Leu, Sy Jye. / Reduced expression of circadian clock genes in male alcoholic patients. 於: Alcoholism: Clinical and Experimental Research. 2010 ; 卷 34, 編號 11. 頁 1899-1904.
@article{106a80678b584988ac8fa7c771d4b759,
title = "Reduced expression of circadian clock genes in male alcoholic patients",
abstract = "Background: There are clear interactions between chronic alcohol consumption and circadian rhythmicity that is regulated by several circadian clock genes. The altered expressions of these genes have been mainly described in animals. The mammalian master clock in the suprachiasmatic nuclei orchestrates the biological rhythms in peripheral tissues. As peripheral blood mononuclear cells (PBMCs) are the most accessible tissue clinically, we assessed the mRNA levels of these genes in patients with alcohol dependence (AD) undergoing alcohol-withdrawal (AW) treatment.Methods: Twenty-two male patients fulfilled the DSM-IV diagnostic criteria of AD, and 12 comparison healthy control subjects were recruited. The patients with AD were further divided by the presence of delirium tremens (DTs), the most severe form of AW syndrome, into DT group and non-DT group. All the participants received blood withdrawal at 9 am, while the patients with AD had blood collection twice: on the next morning of admission (baseline) and on the seventh day. PBMCs were isolated from whole blood, and the mRNA expression profiles of hClock1, hBmal1, hPer1, hPer2, hCry1, and hCry2 were determined by quantitative real-time PCR.Results: The baseline mRNA levels of the target circadian clock genes were markedly lower in patients with AD than in control subjects. After 1 week of alcohol detoxification, there were very limited restorations of discrete circadian gene expressions. DT group did not differ in the expression patterns of circadian clock genes from non-DT group.Conclusions: This is the first study demonstrating the overall lowering of circadian clock genes among patients with AD. The expression pattern is comparable between patients with and without DTs. Although preliminary with data at only one single time point, the observation of strikingly reduced mRNA levels supports the association between circadian clock gene dysregulation and chronic alcohol intake.",
keywords = "Alcohol dependence, Alcohol withdrawal, Circadian clock gene, Circadian rhythm",
author = "Huang, {Ming Chyi} and Ho, {Chia Wei} and Chen, {Chun Hsin} and Liu, {Shing Cheng} and Chen, {Chiao Chicy} and Leu, {Sy Jye}",
year = "2010",
month = "11",
doi = "10.1111/j.1530-0277.2010.01278.x",
language = "English",
volume = "34",
pages = "1899--1904",
journal = "Alcoholism: Clinical and Experimental Research",
issn = "0145-6008",
publisher = "Wiley-Blackwell",
number = "11",

}

TY - JOUR

T1 - Reduced expression of circadian clock genes in male alcoholic patients

AU - Huang, Ming Chyi

AU - Ho, Chia Wei

AU - Chen, Chun Hsin

AU - Liu, Shing Cheng

AU - Chen, Chiao Chicy

AU - Leu, Sy Jye

PY - 2010/11

Y1 - 2010/11

N2 - Background: There are clear interactions between chronic alcohol consumption and circadian rhythmicity that is regulated by several circadian clock genes. The altered expressions of these genes have been mainly described in animals. The mammalian master clock in the suprachiasmatic nuclei orchestrates the biological rhythms in peripheral tissues. As peripheral blood mononuclear cells (PBMCs) are the most accessible tissue clinically, we assessed the mRNA levels of these genes in patients with alcohol dependence (AD) undergoing alcohol-withdrawal (AW) treatment.Methods: Twenty-two male patients fulfilled the DSM-IV diagnostic criteria of AD, and 12 comparison healthy control subjects were recruited. The patients with AD were further divided by the presence of delirium tremens (DTs), the most severe form of AW syndrome, into DT group and non-DT group. All the participants received blood withdrawal at 9 am, while the patients with AD had blood collection twice: on the next morning of admission (baseline) and on the seventh day. PBMCs were isolated from whole blood, and the mRNA expression profiles of hClock1, hBmal1, hPer1, hPer2, hCry1, and hCry2 were determined by quantitative real-time PCR.Results: The baseline mRNA levels of the target circadian clock genes were markedly lower in patients with AD than in control subjects. After 1 week of alcohol detoxification, there were very limited restorations of discrete circadian gene expressions. DT group did not differ in the expression patterns of circadian clock genes from non-DT group.Conclusions: This is the first study demonstrating the overall lowering of circadian clock genes among patients with AD. The expression pattern is comparable between patients with and without DTs. Although preliminary with data at only one single time point, the observation of strikingly reduced mRNA levels supports the association between circadian clock gene dysregulation and chronic alcohol intake.

AB - Background: There are clear interactions between chronic alcohol consumption and circadian rhythmicity that is regulated by several circadian clock genes. The altered expressions of these genes have been mainly described in animals. The mammalian master clock in the suprachiasmatic nuclei orchestrates the biological rhythms in peripheral tissues. As peripheral blood mononuclear cells (PBMCs) are the most accessible tissue clinically, we assessed the mRNA levels of these genes in patients with alcohol dependence (AD) undergoing alcohol-withdrawal (AW) treatment.Methods: Twenty-two male patients fulfilled the DSM-IV diagnostic criteria of AD, and 12 comparison healthy control subjects were recruited. The patients with AD were further divided by the presence of delirium tremens (DTs), the most severe form of AW syndrome, into DT group and non-DT group. All the participants received blood withdrawal at 9 am, while the patients with AD had blood collection twice: on the next morning of admission (baseline) and on the seventh day. PBMCs were isolated from whole blood, and the mRNA expression profiles of hClock1, hBmal1, hPer1, hPer2, hCry1, and hCry2 were determined by quantitative real-time PCR.Results: The baseline mRNA levels of the target circadian clock genes were markedly lower in patients with AD than in control subjects. After 1 week of alcohol detoxification, there were very limited restorations of discrete circadian gene expressions. DT group did not differ in the expression patterns of circadian clock genes from non-DT group.Conclusions: This is the first study demonstrating the overall lowering of circadian clock genes among patients with AD. The expression pattern is comparable between patients with and without DTs. Although preliminary with data at only one single time point, the observation of strikingly reduced mRNA levels supports the association between circadian clock gene dysregulation and chronic alcohol intake.

KW - Alcohol dependence

KW - Alcohol withdrawal

KW - Circadian clock gene

KW - Circadian rhythm

UR - http://www.scopus.com/inward/record.url?scp=78049242505&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78049242505&partnerID=8YFLogxK

U2 - 10.1111/j.1530-0277.2010.01278.x

DO - 10.1111/j.1530-0277.2010.01278.x

M3 - Article

VL - 34

SP - 1899

EP - 1904

JO - Alcoholism: Clinical and Experimental Research

JF - Alcoholism: Clinical and Experimental Research

SN - 0145-6008

IS - 11

ER -