Recruitment by SDF-1α of CD34-positive cells involved in sciatic nerve regeneration: Laboratory investigation

Meei Ling Sheu, Fu Chou Cheng, Hong Lin Su, Ying Ju Chen, Chun Jung Chen, Chih Ming Chiang, Wen Ta Chiu, Jason Sheehan, Hung Chuan Pan

研究成果: 雜誌貢獻文章

11 引文 (Scopus)

摘要

Object. Increased integration of CD34 + cells in injured nerve significantly promotes nerve regeneration, but this effect can be counteracted by limited migration and short survival of CD34 + cells. SDF-1α and its receptor mediate the recruitment of CD34 + cells involved in the repair mechanism of several neurological diseases. In this study, the authors investigate the potentiation of CD34 + cell recruitment triggered by SDF-1α and the involvement of CD34 +cells in peripheral nerve regeneration. Methods. Peripheral nerve injury was induced in 147 Sprague-Dawley rats by crushing the left sciatic nerve with a vessel clamp. The animals were allocated to 3 groups: Group 1, crush injury (controls); Group 2, crush injury and local application of SDF-1α recombinant proteins; and Group 3, crush injury and local application of SDF-1α antibody. Electrophysiological studies and assessment of regeneration markers were conducted at 4 weeks after injury; neurobehavioral studies were conducted at 1, 2, 3, and 4 weeks after injury. The expression of SDF-1α, accumulation of CD34 + cells, immune cells, and angiogenesis factors in injured nerves were evaluated at 1, 3, 7, 10, 14, 21, and 28 days after injury. Results. Application of SDF-1α increased the migration of CD34 + cells in vitro, and this effect was dose dependent. Crush injury induced the expression of SDF-1α, with a peak of 10-14 days postinjury, and this increased expression of SDF-1α paralleled the deposition of CD34 + cells, expression of VEGF, and expression of neurofilament. These effects were further enhanced by the administration of SDF-1α recombinant protein and abolished by administration of SDF-1α antibody. Furthermore, these effects were consistent with improvement in measures of neurological function such as sciatic function index, electrophysiological parameters, muscle weight, and myelination of regenerative nerve. Conclusions. Expression of SDF-1α facilitates recruitment of CD34 + cells in peripheral nerve injury. The increased deposition of CD34 + cells paralleled significant expression of angiogenesis factors and was consistent with improvement of neurological function. Utilization of SDF-1α for enhancing the recruitment of CD34 + cells involved in peripheral nerve regeneration may be considered as an alternative treatment strategy in peripheral nerve disorders.

原文英語
頁(從 - 到)432-444
頁數13
期刊Journal of Neurosurgery
116
發行號2
DOIs
出版狀態已發佈 - 二月 2012

指紋

Nerve Regeneration
Sciatic Nerve
Peripheral Nerves
Peripheral Nerve Injuries
Angiogenesis Inducing Agents
Recombinant Proteins
Wounds and Injuries
Intermediate Filaments
Antibodies
Immunologic Factors
Vascular Endothelial Growth Factor A
Cell Movement
Sprague Dawley Rats
Regeneration
Cell Survival
Weights and Measures
Muscles

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

引用此文

Sheu, M. L., Cheng, F. C., Su, H. L., Chen, Y. J., Chen, C. J., Chiang, C. M., ... Pan, H. C. (2012). Recruitment by SDF-1α of CD34-positive cells involved in sciatic nerve regeneration: Laboratory investigation. Journal of Neurosurgery, 116(2), 432-444. https://doi.org/10.3171/2011.3.JNS101582

Recruitment by SDF-1α of CD34-positive cells involved in sciatic nerve regeneration : Laboratory investigation. / Sheu, Meei Ling; Cheng, Fu Chou; Su, Hong Lin; Chen, Ying Ju; Chen, Chun Jung; Chiang, Chih Ming; Chiu, Wen Ta; Sheehan, Jason; Pan, Hung Chuan.

於: Journal of Neurosurgery, 卷 116, 編號 2, 02.2012, p. 432-444.

研究成果: 雜誌貢獻文章

Sheu, ML, Cheng, FC, Su, HL, Chen, YJ, Chen, CJ, Chiang, CM, Chiu, WT, Sheehan, J & Pan, HC 2012, 'Recruitment by SDF-1α of CD34-positive cells involved in sciatic nerve regeneration: Laboratory investigation', Journal of Neurosurgery, 卷 116, 編號 2, 頁 432-444. https://doi.org/10.3171/2011.3.JNS101582
Sheu, Meei Ling ; Cheng, Fu Chou ; Su, Hong Lin ; Chen, Ying Ju ; Chen, Chun Jung ; Chiang, Chih Ming ; Chiu, Wen Ta ; Sheehan, Jason ; Pan, Hung Chuan. / Recruitment by SDF-1α of CD34-positive cells involved in sciatic nerve regeneration : Laboratory investigation. 於: Journal of Neurosurgery. 2012 ; 卷 116, 編號 2. 頁 432-444.
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abstract = "Object. Increased integration of CD34 + cells in injured nerve significantly promotes nerve regeneration, but this effect can be counteracted by limited migration and short survival of CD34 + cells. SDF-1α and its receptor mediate the recruitment of CD34 + cells involved in the repair mechanism of several neurological diseases. In this study, the authors investigate the potentiation of CD34 + cell recruitment triggered by SDF-1α and the involvement of CD34 +cells in peripheral nerve regeneration. Methods. Peripheral nerve injury was induced in 147 Sprague-Dawley rats by crushing the left sciatic nerve with a vessel clamp. The animals were allocated to 3 groups: Group 1, crush injury (controls); Group 2, crush injury and local application of SDF-1α recombinant proteins; and Group 3, crush injury and local application of SDF-1α antibody. Electrophysiological studies and assessment of regeneration markers were conducted at 4 weeks after injury; neurobehavioral studies were conducted at 1, 2, 3, and 4 weeks after injury. The expression of SDF-1α, accumulation of CD34 + cells, immune cells, and angiogenesis factors in injured nerves were evaluated at 1, 3, 7, 10, 14, 21, and 28 days after injury. Results. Application of SDF-1α increased the migration of CD34 + cells in vitro, and this effect was dose dependent. Crush injury induced the expression of SDF-1α, with a peak of 10-14 days postinjury, and this increased expression of SDF-1α paralleled the deposition of CD34 + cells, expression of VEGF, and expression of neurofilament. These effects were further enhanced by the administration of SDF-1α recombinant protein and abolished by administration of SDF-1α antibody. Furthermore, these effects were consistent with improvement in measures of neurological function such as sciatic function index, electrophysiological parameters, muscle weight, and myelination of regenerative nerve. Conclusions. Expression of SDF-1α facilitates recruitment of CD34 + cells in peripheral nerve injury. The increased deposition of CD34 + cells paralleled significant expression of angiogenesis factors and was consistent with improvement of neurological function. Utilization of SDF-1α for enhancing the recruitment of CD34 + cells involved in peripheral nerve regeneration may be considered as an alternative treatment strategy in peripheral nerve disorders.",
keywords = "Hematopoietic progenitor cell, Nerve regeneration, Peripheral nerve, Sciatic nerve crush injury, SDF-1α",
author = "Sheu, {Meei Ling} and Cheng, {Fu Chou} and Su, {Hong Lin} and Chen, {Ying Ju} and Chen, {Chun Jung} and Chiang, {Chih Ming} and Chiu, {Wen Ta} and Jason Sheehan and Pan, {Hung Chuan}",
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T2 - Laboratory investigation

AU - Sheu, Meei Ling

AU - Cheng, Fu Chou

AU - Su, Hong Lin

AU - Chen, Ying Ju

AU - Chen, Chun Jung

AU - Chiang, Chih Ming

AU - Chiu, Wen Ta

AU - Sheehan, Jason

AU - Pan, Hung Chuan

PY - 2012/2

Y1 - 2012/2

N2 - Object. Increased integration of CD34 + cells in injured nerve significantly promotes nerve regeneration, but this effect can be counteracted by limited migration and short survival of CD34 + cells. SDF-1α and its receptor mediate the recruitment of CD34 + cells involved in the repair mechanism of several neurological diseases. In this study, the authors investigate the potentiation of CD34 + cell recruitment triggered by SDF-1α and the involvement of CD34 +cells in peripheral nerve regeneration. Methods. Peripheral nerve injury was induced in 147 Sprague-Dawley rats by crushing the left sciatic nerve with a vessel clamp. The animals were allocated to 3 groups: Group 1, crush injury (controls); Group 2, crush injury and local application of SDF-1α recombinant proteins; and Group 3, crush injury and local application of SDF-1α antibody. Electrophysiological studies and assessment of regeneration markers were conducted at 4 weeks after injury; neurobehavioral studies were conducted at 1, 2, 3, and 4 weeks after injury. The expression of SDF-1α, accumulation of CD34 + cells, immune cells, and angiogenesis factors in injured nerves were evaluated at 1, 3, 7, 10, 14, 21, and 28 days after injury. Results. Application of SDF-1α increased the migration of CD34 + cells in vitro, and this effect was dose dependent. Crush injury induced the expression of SDF-1α, with a peak of 10-14 days postinjury, and this increased expression of SDF-1α paralleled the deposition of CD34 + cells, expression of VEGF, and expression of neurofilament. These effects were further enhanced by the administration of SDF-1α recombinant protein and abolished by administration of SDF-1α antibody. Furthermore, these effects were consistent with improvement in measures of neurological function such as sciatic function index, electrophysiological parameters, muscle weight, and myelination of regenerative nerve. Conclusions. Expression of SDF-1α facilitates recruitment of CD34 + cells in peripheral nerve injury. The increased deposition of CD34 + cells paralleled significant expression of angiogenesis factors and was consistent with improvement of neurological function. Utilization of SDF-1α for enhancing the recruitment of CD34 + cells involved in peripheral nerve regeneration may be considered as an alternative treatment strategy in peripheral nerve disorders.

AB - Object. Increased integration of CD34 + cells in injured nerve significantly promotes nerve regeneration, but this effect can be counteracted by limited migration and short survival of CD34 + cells. SDF-1α and its receptor mediate the recruitment of CD34 + cells involved in the repair mechanism of several neurological diseases. In this study, the authors investigate the potentiation of CD34 + cell recruitment triggered by SDF-1α and the involvement of CD34 +cells in peripheral nerve regeneration. Methods. Peripheral nerve injury was induced in 147 Sprague-Dawley rats by crushing the left sciatic nerve with a vessel clamp. The animals were allocated to 3 groups: Group 1, crush injury (controls); Group 2, crush injury and local application of SDF-1α recombinant proteins; and Group 3, crush injury and local application of SDF-1α antibody. Electrophysiological studies and assessment of regeneration markers were conducted at 4 weeks after injury; neurobehavioral studies were conducted at 1, 2, 3, and 4 weeks after injury. The expression of SDF-1α, accumulation of CD34 + cells, immune cells, and angiogenesis factors in injured nerves were evaluated at 1, 3, 7, 10, 14, 21, and 28 days after injury. Results. Application of SDF-1α increased the migration of CD34 + cells in vitro, and this effect was dose dependent. Crush injury induced the expression of SDF-1α, with a peak of 10-14 days postinjury, and this increased expression of SDF-1α paralleled the deposition of CD34 + cells, expression of VEGF, and expression of neurofilament. These effects were further enhanced by the administration of SDF-1α recombinant protein and abolished by administration of SDF-1α antibody. Furthermore, these effects were consistent with improvement in measures of neurological function such as sciatic function index, electrophysiological parameters, muscle weight, and myelination of regenerative nerve. Conclusions. Expression of SDF-1α facilitates recruitment of CD34 + cells in peripheral nerve injury. The increased deposition of CD34 + cells paralleled significant expression of angiogenesis factors and was consistent with improvement of neurological function. Utilization of SDF-1α for enhancing the recruitment of CD34 + cells involved in peripheral nerve regeneration may be considered as an alternative treatment strategy in peripheral nerve disorders.

KW - Hematopoietic progenitor cell

KW - Nerve regeneration

KW - Peripheral nerve

KW - Sciatic nerve crush injury

KW - SDF-1α

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