Recombinational resolution in primate cells of two homologous human DNA segments with a gradient of sequence divergence

Manza Gomez-Pedrozo, Wei Shau Hu, Che Kun James Shen

研究成果: 雜誌貢獻文章同行評審

1 引文 斯高帕斯(Scopus)

摘要

Human α-thalassemia-2 genotype -α4.2 is the result of meiotic recombination between two 1.3 kb long, homologous DNA segments, X(α2) and X(α1), located in the adult α globin locus. The two segments can also undergo intramolecular recombination on extrachromosomal vectors transfected into mitoically dividing primate cells (COS 7). The existence of a gradient of sequence divergence between X(α2) and X(α1) makes them an interesting system to study the relationship between efficiencies of homologous DNA recombinaiion and the extent of dispersed and localized base mismatches. By partial restriction mapping and DNA sequencing of plasmids recombined in COS 7 cells and rescued from bacteria HB 101, we have determined the distribution of recombinational resolution sites along the two X blocks. Most, if not all, of the homologous recombination events between the two X blocks appear to be single crossing-over without efficient gene correction or repair of base mismatches. The distribution of the sites of recombinational resolution is inversely correlated with that of the gradient of sequence divergence, with only approximately 7% of the X recombinants resolved within the 3′ third of the X blocks where two diverged Alu family repeats reside. The Alu sequence within which one of the X recombinants resolved is homologous to a previously characterized α thalassemia deletion point.

原文英語
頁(從 - 到)11237-11247
頁數11
期刊Nucleic Acids Research
16
發行號23
DOIs
出版狀態已發佈 - 十二月 9 1988
對外發佈

ASJC Scopus subject areas

  • 遺傳學

指紋

深入研究「Recombinational resolution in primate cells of two homologous human DNA segments with a gradient of sequence divergence」主題。共同形成了獨特的指紋。

引用此