Recognition of the three deduced probable HLA haplotypes that areassociated with HLA-C*16: 04:01 (A*33:03-B*44:02-C*16:04:01-DRB1*11:04:01 and A*24-B*44:02-C*16:04:01-DRB1*11:04) and HLA-B*15:109 (A*11-B*15:109-DRB1*04) in Taiwanese unrelated hematopoietic stem cell donors

Kuo Liang Yang, Reuy Ho Kao, Chin Lon Lin, Py Yu Lin

研究成果: 雜誌貢獻文章

10 引文 斯高帕斯(Scopus)

摘要

Objective: HLA-C*16:04:01 and HLA-B*15:109 are two uncommon alleles at the HLA-C locus and HLA-B locus, respectively. The objective of this study is to report the deduced probable human leukocyte antigen (HLA) haplotypes associated with HLA-C*16:04:01 and HLA-B*15:109 among Taiwanese unrelated bone marrow hematopoietic stem cell donors. Materials and methods: A sequence-based typing method was employed to confirm the two low incidence alleles observed. Polymerase chain reaction was performed to amplify exons 2 and 3 of the HLA-A, and HLA-B loci with group-specific primer sets. Amplicons were sequenced using a BigDye Terminator Cycle Sequencing Ready Reaction Kit in both directions according to the manufacturer's protocols. Results: The DNA sequence of C*16:04:01 is identical to that of C*16:01:01 in exons 2 and 3, except for two nucleotide substitutions at residues 538 (C->T) and 539 (A->G), which results in a single amino acid replacement at position 156 (glutamine->tryptophan). We deduced two probable HLA haplotypes that are found in association with C*16:04:01 as A*33:03-B*44:02-C*16:04:01-DRB1*11:04 and A*24-B*44:02-C*16:04:01-DRB1*11:04. The DNA sequence of B*15:109 is identical to B*15:27:01 in exons 2 and 3 except for one nucleotide substitution at residue 200 (C->T), which results in a single amino acid replacement at position 43 (proline->leucine). A probable HLA haplotype associated with B*15:109 was deduced to be A*11-B*15:109-DRB1*04. Conclusion: Information on the deduced HLA haplotypes that are found in association with the rare C*16:04:01 and B*15:109 alleles that we report here is useful for reference purposes at HLA testing laboratories and will help stem cell transplantation donor search coordinators when they are determining the likelihood of finding a compatible donor for patients bearing these two uncommon HLA alleles from unrelated bone marrow donor registries.
原文英語
頁(從 - 到)123-126
頁數4
期刊Tzu Chi Medical Journal
26
發行號3
DOIs
出版狀態已發佈 - 一月 1 2014
對外發佈Yes

ASJC Scopus subject areas

  • Medicine(all)

指紋 深入研究「Recognition of the three deduced probable HLA haplotypes that areassociated with HLA-C*16: 04:01 (A*33:03-B*44:02-C*16:04:01-DRB1*11:04:01 and A*24-B*44:02-C*16:04:01-DRB1*11:04) and HLA-B*15:109 (A*11-B*15:109-DRB1*04) in Taiwanese unrelated hematopoietic stem cell donors」主題。共同形成了獨特的指紋。

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