Receptor guanylyl cyclase-G is a novel thermosensory protein activated by cool temperatures

Ying Chi Chao, Chih Cheng Chen, Yuh Charn Lin, Heinz Breer, Joerg Fleischer, Ruey Bing Yang

研究成果: 雜誌貢獻文章同行評審

31 引文 斯高帕斯(Scopus)

摘要

Transmembrane guanylyl cyclases (GCs), with activity regulated by peptide ligands and/or calcium-binding proteins, are essential for various physiological and sensory processes. The mode of activation of the GC subtype GC-G, which is expressed in neurons of the Grueneberg ganglion that respond to cool temperatures, has been elusive. In searching for appropriate stimuli to activate GC-G, we found that its enzymatic activity is directly stimulated by cool temperatures. In this context, it was observed that dimerization/oligomerization of GC-G, a process generally considered as critical for enzymatic activity of GCs, is strongly enhanced by coolness. Moreover, heterologous expression of GC-G in cultured cells rendered these cells responsive to coolness; thus, the protein might be a sensor for cool temperatures. This concept is supported by the observation of substantially reduced coolness-induced response of Grueneberg ganglion neurons and coolness-evoked ultrasonic vocalization in GC-G-deficient mouse pups. GC-G may be a novel thermosensory protein with functional implications for the Grueneberg ganglion, a sensory organ responding to cool temperatures.
原文英語
頁(從 - 到)294-306
頁數13
期刊EMBO Journal
34
發行號3
DOIs
出版狀態已發佈 - 2月 3 2015

ASJC Scopus subject areas

  • 神經科學 (全部)
  • 分子生物學
  • 生物化學、遺傳與分子生物學 (全部)
  • 免疫學與微生物學 (全部)

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