Recapitulation of zebrafish sncga expression pattern and labeling the habenular complex in transgenic zebrafish using green fluorescent protein reporter gene

Yi Chung Chen, Chia Hsiung Cheng, Gen Der Chen, Chin Chun Hung, Chung Hsiang Yang, Sheng Ping L Hwang, Koichi Kawakami, Bo Kai Wu, Chang Jen Huang

研究成果: 雜誌貢獻文章

22 引文 (Scopus)

摘要

Human synuclein family consists of α-, β-, and γ-synucleins. Here, we cloned three genes, sncb, sncga and sncgb from zebrafish. They encode β-, γ1-, and γ2-synucleins, respectively. The zSyn-γ, zSyn-γ1, and zSyn-γ2 proteins display 69%, 47%, and 50% identity to human β-synuclein and γ-synuclein, respectively. By reverse transcriptase-polymerase chain reaction, we demonstrated that sncb and sncga mRNA were abundant in brain and eye, while sncgb expression was moderate in brain, kidney, ovary and testis. The 1.8-kb 5′-upstream/promoter region of the sncga gene was sufficient to direct green fluorescent protein (GFP) expression in the central nervous system and cranial ganglions. A transgenic line, Tg(sncga:GFP), was generated and its GFP expression is similar to that of endogenous sncga mRNA. Moreover, this line also labels the habenular complex and the domain of GFP expression is larger in the left than in the right habenula. Thus, this line can be used to study sncga gene regulation and for left-right asymmetry study in zebrafish brain.

原文英語
頁(從 - 到)746-754
頁數9
期刊Developmental Dynamics
238
發行號3
DOIs
出版狀態已發佈 - 三月 2009
對外發佈Yes

指紋

Synucleins
Zebrafish
Green Fluorescent Proteins
Reporter Genes
Brain
Habenula
Genes
Messenger RNA
Reverse Transcriptase Polymerase Chain Reaction
Genetic Promoter Regions
Ganglia
Testis
Ovary
Central Nervous System
Kidney
Proteins

ASJC Scopus subject areas

  • Developmental Biology

引用此文

Recapitulation of zebrafish sncga expression pattern and labeling the habenular complex in transgenic zebrafish using green fluorescent protein reporter gene. / Chen, Yi Chung; Cheng, Chia Hsiung; Chen, Gen Der; Hung, Chin Chun; Yang, Chung Hsiang; Hwang, Sheng Ping L; Kawakami, Koichi; Wu, Bo Kai; Huang, Chang Jen.

於: Developmental Dynamics, 卷 238, 編號 3, 03.2009, p. 746-754.

研究成果: 雜誌貢獻文章

Chen, Yi Chung ; Cheng, Chia Hsiung ; Chen, Gen Der ; Hung, Chin Chun ; Yang, Chung Hsiang ; Hwang, Sheng Ping L ; Kawakami, Koichi ; Wu, Bo Kai ; Huang, Chang Jen. / Recapitulation of zebrafish sncga expression pattern and labeling the habenular complex in transgenic zebrafish using green fluorescent protein reporter gene. 於: Developmental Dynamics. 2009 ; 卷 238, 編號 3. 頁 746-754.
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abstract = "Human synuclein family consists of α-, β-, and γ-synucleins. Here, we cloned three genes, sncb, sncga and sncgb from zebrafish. They encode β-, γ1-, and γ2-synucleins, respectively. The zSyn-γ, zSyn-γ1, and zSyn-γ2 proteins display 69{\%}, 47{\%}, and 50{\%} identity to human β-synuclein and γ-synuclein, respectively. By reverse transcriptase-polymerase chain reaction, we demonstrated that sncb and sncga mRNA were abundant in brain and eye, while sncgb expression was moderate in brain, kidney, ovary and testis. The 1.8-kb 5′-upstream/promoter region of the sncga gene was sufficient to direct green fluorescent protein (GFP) expression in the central nervous system and cranial ganglions. A transgenic line, Tg(sncga:GFP), was generated and its GFP expression is similar to that of endogenous sncga mRNA. Moreover, this line also labels the habenular complex and the domain of GFP expression is larger in the left than in the right habenula. Thus, this line can be used to study sncga gene regulation and for left-right asymmetry study in zebrafish brain.",
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AU - Hung, Chin Chun

AU - Yang, Chung Hsiang

AU - Hwang, Sheng Ping L

AU - Kawakami, Koichi

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AU - Huang, Chang Jen

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