Real-time detection of β1 integrin expression on MG-63 cells using electrochemical impedance spectroscopy

Chun Yen Lin, Nai Chia Teng, Sung Chih Hsieh, Yung Sheng Lin, Wei Jen Chang, Sheng Yi Hsiao, Hui Shun Huang, Haw Ming Huang

研究成果: 雜誌貢獻文章同行評審

7 引文 斯高帕斯(Scopus)

摘要

Beta 1 integrin is a membrane protein responsible for attachment and migration of osteosarcoma cells. In this study, expression of β1 integrin on MG-63 cells, a human osteogenic sarcoma cell line, was monitored using electrochemical impedance spectroscopy (EIS). ITO-based biochips were developed using a semiconductor technique. Differences in electric resistance (ΔR) were measured continuously when cells binding with anti-β1 integrin antibody coagulated with nano-scale gold particles. The results of the EIS system were compared with traditional immunofluorescence staining. We found that sample chambers with higher cell densities had larger ΔR values. When the cell densities increased from 5×10 4 cells/ml to 5×10 5 cells/ml, the ΔR value dose-dependently increased from 14Ω to 37Ω. In addition, a highly linear relationship (correlation coefficient, 0.921) was found between the ΔR values and the corresponding fluorescence intensities (p
原文英語
頁(從 - 到)221-226
頁數6
期刊Biosensors and Bioelectronics
28
發行號1
DOIs
出版狀態已發佈 - 十月 15 2011

ASJC Scopus subject areas

  • 生物物理學
  • 生物醫學工程
  • 生物技術
  • 電化學

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