RBM4 promotes pancreas cell differentiation and insulin expression

Jung Chun Lin, Yu Ting Yan, Wen Kou Hsieh, Pey Jey Peng, Chun Hao Su, Woan Yuh Tarna

研究成果: 雜誌貢獻文章同行評審

33 引文 斯高帕斯(Scopus)


The RNA-binding protein RNA-binding motif protein 4 (RBM4) modulates alternative splicing of muscle-specific mRNA isoforms during muscle cell differentiation. To better understand the physiological function of RBM4, we exploited a gene knockout strategy in the present study. Mice with targeted disruption of one of the two Rbm4 genes exhibited hyperglycemia coincident with reduced levels of serum insulin and reduced size of pancreatic islets. The embryonic pancreases of Rbm4-deficient mice showed reduced expression or aberrant splicing of many transcripts encoding factors required for pancreas cell differentiation and function. Using pancreatic acinar AR42J cells, we demonstrated that RBM4 promoted insulin gene expression by altering the isoform balance of the transcription factors Isl1 and Pax4 via alternative splicing control. RBM4 overexpression was sufficient to convert AR42J cells into insulin-producing cells. Moreover, RBM4 may mediate glucose-induced insulin expression and insulin receptor isoform switches. These results suggest that RBM4 may have role in promoting pancreas cell differentiation and endocrine function, essentially via alternative splicing regulation.

頁(從 - 到)319-327
期刊Molecular and Cellular Biology
出版狀態已發佈 - 1月 2013

ASJC Scopus subject areas

  • 分子生物學
  • 細胞生物學


深入研究「RBM4 promotes pancreas cell differentiation and insulin expression」主題。共同形成了獨特的指紋。