Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean

Ching Yu Lin, Cheng Chun Kuan, Rong Huay Juang, Samson C S Tsou, C. George Kuo, Ching San Chen

研究成果: 雜誌貢獻文章

1 引文 斯高帕斯(Scopus)

摘要

We describe an application of cDNA libraries constructed from total RNA to isolate simultaneously many differentially expressed cDNAs between a pair of near isogenic lines of mungbean in a short period of time. All ten selected individual cDNA clones, containing inserts ranging from 1.1-1.9 kb, clearly showed positive Northern blotting. Nucleotide sequencing indicated that six of the clones were probably full-length cDNAs.
原文英語
頁(從 - 到)87-91
頁數5
期刊Botanical Bulletin of Academia Sinica
39
發行號2
出版狀態已發佈 - 1998
對外發佈Yes

    指紋

ASJC Scopus subject areas

  • Plant Science

引用此

Lin, C. Y., Kuan, C. C., Juang, R. H., Tsou, S. C. S., Kuo, C. G., & Chen, C. S. (1998). Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean. Botanical Bulletin of Academia Sinica, 39(2), 87-91.