Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean

Ching Yu Lin, Cheng Chun Kuan, Rong Huay Juang, Samson C S Tsou, C. George Kuo, Ching San Chen

研究成果: 雜誌貢獻文章

1 引文 (Scopus)

摘要

We describe an application of cDNA libraries constructed from total RNA to isolate simultaneously many differentially expressed cDNAs between a pair of near isogenic lines of mungbean in a short period of time. All ten selected individual cDNA clones, containing inserts ranging from 1.1-1.9 kb, clearly showed positive Northern blotting. Nucleotide sequencing indicated that six of the clones were probably full-length cDNAs.
原文英語
頁(從 - 到)87-91
頁數5
期刊Botanical Bulletin of Academia Sinica
39
發行號2
出版狀態已發佈 - 1998
對外發佈Yes

指紋

isogenic lines
mung beans
clones
cDNA libraries
Northern blotting
nucleotides
RNA

ASJC Scopus subject areas

  • Plant Science

引用此文

Lin, C. Y., Kuan, C. C., Juang, R. H., Tsou, S. C. S., Kuo, C. G., & Chen, C. S. (1998). Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean. Botanical Bulletin of Academia Sinica, 39(2), 87-91.

Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean. / Lin, Ching Yu; Kuan, Cheng Chun; Juang, Rong Huay; Tsou, Samson C S; Kuo, C. George; Chen, Ching San.

於: Botanical Bulletin of Academia Sinica, 卷 39, 編號 2, 1998, p. 87-91.

研究成果: 雜誌貢獻文章

Lin, CY, Kuan, CC, Juang, RH, Tsou, SCS, Kuo, CG & Chen, CS 1998, 'Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean', Botanical Bulletin of Academia Sinica, 卷 39, 編號 2, 頁 87-91.
Lin, Ching Yu ; Kuan, Cheng Chun ; Juang, Rong Huay ; Tsou, Samson C S ; Kuo, C. George ; Chen, Ching San. / Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean. 於: Botanical Bulletin of Academia Sinica. 1998 ; 卷 39, 編號 2. 頁 87-91.
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