Rapid detection and biovar differentiation of Ureaplasma urealyticum in clinical specimens by PCR.

L. J. Teng, S. W. Ho, H. N. Ho, S. J. Liaw, H. C. Lai, Kwen Tay Luh

研究成果: 雜誌貢獻文章

15 引文 (Scopus)

摘要

On the basis of the nucleotide sequence of the multiple-banded (MB) antigen genes of Ureaplasma urealyticum, a polymerase chain reaction (PCR) technique was developed for rapid detection and biovar differentiation of U. urealyticum in a total of 100 urogenital specimens from 50 female patients. Positive PCR UM-1 amplification was found in 28 cervical swabs and 31 urine samples. Overall agreement between PCR and culture was 95%. Members of the two biovars of U. urealyticum could be distinguished by the size of the PCR UM-1 amplification products. Biovar differentiation was also demonstrated by two additional sets of PCRs: PCR UM-2 and UM-3. The PCR UM-2 was used to amplify biovar 1, while PCR UM-3 amplified biovar 2 specifically. The results indicated that use of the MB antigen gene as a target for PCR amplification could provide rapid and specific detection and biotyping of ureaplasma DNA in urogenital samples.
原文英語
頁(從 - 到)396-400
頁數5
期刊Journal of the Formosan Medical Association = Taiwan yi zhi
94
發行號7
出版狀態已發佈 - 一月 1 1995
對外發佈Yes

指紋

Molecular Sequence Data
Bacterial Typing Techniques
Ureaplasma urealyticum
Polymerase Chain Reaction
Ureaplasma
Antigens
Genes

ASJC Scopus subject areas

  • Medicine(all)

引用此文

Rapid detection and biovar differentiation of Ureaplasma urealyticum in clinical specimens by PCR. / Teng, L. J.; Ho, S. W.; Ho, H. N.; Liaw, S. J.; Lai, H. C.; Luh, Kwen Tay.

於: Journal of the Formosan Medical Association = Taiwan yi zhi, 卷 94, 編號 7, 01.01.1995, p. 396-400.

研究成果: 雜誌貢獻文章

Teng, L. J. ; Ho, S. W. ; Ho, H. N. ; Liaw, S. J. ; Lai, H. C. ; Luh, Kwen Tay. / Rapid detection and biovar differentiation of Ureaplasma urealyticum in clinical specimens by PCR. 於: Journal of the Formosan Medical Association = Taiwan yi zhi. 1995 ; 卷 94, 編號 7. 頁 396-400.
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abstract = "On the basis of the nucleotide sequence of the multiple-banded (MB) antigen genes of Ureaplasma urealyticum, a polymerase chain reaction (PCR) technique was developed for rapid detection and biovar differentiation of U. urealyticum in a total of 100 urogenital specimens from 50 female patients. Positive PCR UM-1 amplification was found in 28 cervical swabs and 31 urine samples. Overall agreement between PCR and culture was 95{\%}. Members of the two biovars of U. urealyticum could be distinguished by the size of the PCR UM-1 amplification products. Biovar differentiation was also demonstrated by two additional sets of PCRs: PCR UM-2 and UM-3. The PCR UM-2 was used to amplify biovar 1, while PCR UM-3 amplified biovar 2 specifically. The results indicated that use of the MB antigen gene as a target for PCR amplification could provide rapid and specific detection and biotyping of ureaplasma DNA in urogenital samples.",
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AU - Lai, H. C.

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