Rapid and highly sensitive detection of Enterovirus 71 by using nanogold-enhanced electrochemical impedance spectroscopy

Hsing Yuan Li, Shing Hua Tseng, Tsai Mu Cheng, Hsueh Liang Chu, Yu Ning Lu, Fang Yu Wang, Li Yun Tsai, Juo Yu Shieh, Jyh Yuan Yang, Chien Chang Juan, Lung Chen Tu, Chia Ching Chang

研究成果: 雜誌貢獻文章

3 引文 (Scopus)

摘要

Enterovirus 71 (EV71) infection is an emerging infectious disease causing neurological complications and/or death within two to three days after the development of fever and rash. A low viral titre in clinical specimens makes the detection of EV71 difficult. Conventional approaches for detecting EV71 are time consuming, poorly sensitive, or complicated, and cannot be used effectively for clinical diagnosis. Furthermore, EV71 and Coxsackie virus A16 (CA16) may cross react in conventional assays. Therefore, a rapid, highly sensitive, specific, and user-friendly test is needed. We developed an EV71-specific nanogold-modified working electrode for electrochemical impedance spectroscopy in the detection of EV71. Our results show that EV71 can be distinguished from CA16, Herpes simplex virus, and lysozyme, with the modified nanogold electrode being able to detect EV71 in concentrations as low as 1 copy number/50 μl reaction volume, and the duration between sample preparation and detection being 11 min. This detection platform may have the potential for use in point-of-care diagnostics.
原文英語
文章編號285102
期刊Nanotechnology
24
發行號28
DOIs
出版狀態已發佈 - 七月 19 2013

指紋

Dielectric Spectroscopy
Enterovirus
Electrochemical impedance spectroscopy
Viruses
Electrodes
Muramidase
Assays
Enzymes
Point-of-Care Systems
Enterovirus Infections
Emerging Communicable Diseases
Simplexvirus
Exanthema
Fever

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)
  • Medicine(all)

引用此文

Rapid and highly sensitive detection of Enterovirus 71 by using nanogold-enhanced electrochemical impedance spectroscopy. / Li, Hsing Yuan; Tseng, Shing Hua; Cheng, Tsai Mu; Chu, Hsueh Liang; Lu, Yu Ning; Wang, Fang Yu; Tsai, Li Yun; Shieh, Juo Yu; Yang, Jyh Yuan; Juan, Chien Chang; Tu, Lung Chen; Chang, Chia Ching.

於: Nanotechnology, 卷 24, 編號 28, 285102, 19.07.2013.

研究成果: 雜誌貢獻文章

Li, HY, Tseng, SH, Cheng, TM, Chu, HL, Lu, YN, Wang, FY, Tsai, LY, Shieh, JY, Yang, JY, Juan, CC, Tu, LC & Chang, CC 2013, 'Rapid and highly sensitive detection of Enterovirus 71 by using nanogold-enhanced electrochemical impedance spectroscopy', Nanotechnology, 卷 24, 編號 28, 285102. https://doi.org/10.1088/0957-4484/24/28/285102
Li, Hsing Yuan ; Tseng, Shing Hua ; Cheng, Tsai Mu ; Chu, Hsueh Liang ; Lu, Yu Ning ; Wang, Fang Yu ; Tsai, Li Yun ; Shieh, Juo Yu ; Yang, Jyh Yuan ; Juan, Chien Chang ; Tu, Lung Chen ; Chang, Chia Ching. / Rapid and highly sensitive detection of Enterovirus 71 by using nanogold-enhanced electrochemical impedance spectroscopy. 於: Nanotechnology. 2013 ; 卷 24, 編號 28.
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abstract = "Enterovirus 71 (EV71) infection is an emerging infectious disease causing neurological complications and/or death within two to three days after the development of fever and rash. A low viral titre in clinical specimens makes the detection of EV71 difficult. Conventional approaches for detecting EV71 are time consuming, poorly sensitive, or complicated, and cannot be used effectively for clinical diagnosis. Furthermore, EV71 and Coxsackie virus A16 (CA16) may cross react in conventional assays. Therefore, a rapid, highly sensitive, specific, and user-friendly test is needed. We developed an EV71-specific nanogold-modified working electrode for electrochemical impedance spectroscopy in the detection of EV71. Our results show that EV71 can be distinguished from CA16, Herpes simplex virus, and lysozyme, with the modified nanogold electrode being able to detect EV71 in concentrations as low as 1 copy number/50 μl reaction volume, and the duration between sample preparation and detection being 11 min. This detection platform may have the potential for use in point-of-care diagnostics.",
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