Rapid analysis of melamine in infant formula by sweeping-micellar electrokinetic chromatography

I. L. Tsai, Shao W. Sun, Hsiao W. Liao, Shu Chiao Lin, Ching H. Kuo

研究成果: 雜誌貢獻文章同行評審

69 引文 斯高帕斯(Scopus)

摘要

In the present study, an analytical method using capillary electrophoresis with on-line preconcentration technique was developed for rapid determination of melamine in infant formula. Both stacking and sweeping preconcentration techniques had been investigated for the comparison of their effectiveness in melamine analysis. The limit of detection of melamine standard was 0.5 ng/mL for the field amplified sample stacking (FASS) technique and 9.2 ng/mL for the sweeping technique. Although the FASS technique provided better concentration efficacy than the sweeping technique, the matrix effect was more profound with the former. Matrix effect was evaluated by comparing the enhancement factor (EF) of melamine standard and post-extraction spiked infant formula solution. The EF was changed from 429.86 ± 9.81 to the level less than 133.31 with significant peak distortion in the FASS system, and it was remained unchanged in the sweeping system. Sweeping-micellar electrokinetic chromatography (sweeping-MEKC) was demonstrated to be most suitable for real sample analysis. Under optimum sweeping-MEKC conditions, melamine content in infant formulas could be determined within 6 min. The developed solid phase extraction (SPE) procedures coupled with the sweeping-MEKC method was subjected to method validation. Run-to-run repeatability (n = 3) and day-to-day reproducibility (n = 3) of peak area were within 3.6% and 4.8% RSD, respectively. The accuracy was tested by spiking 0.5 and 2 μg/mL of melamine standard in the melamine contaminated milk powder provided by the European Commission, and the recoveries were 93.4 ± 0.5% and 98.7 ± 0.4%, respectively. Results of this study show a great potential for the sweeping-MEKC method as a tool for the fast screening of melamine in infant formulas.

原文英語
頁(從 - 到)8296-8303
頁數8
期刊Journal of Chromatography A
1216
發行號47
DOIs
出版狀態已發佈 - 11月 20 2009
對外發佈

ASJC Scopus subject areas

  • 生物化學
  • 分析化學
  • 有機化學

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