摘要
In this study, we investigated Lycium barbarum, a Chinese medicine sold on the Taiwan market, using RAPD analysis. Sixty oligonucleotide primers were used to screen twenty randomly selected samples in the analysis. Total DNA extracted from the fruit of the medicine material was used as template in the PCR reaction. Four primers: OPD-15, OPG-15, OPT-12 and OPT-17, showed distinct polymorphic patterns, but others exhibited profiles nearly identical to the other samples used in the study. We found that only two RAPD fingerprinting types of these primers were outlined from twenty collected Lycium samples. Fifteen samples showed the first type of profiles while only five samples resulted of the second type. A low genetic diversity among the Lycium barbarum samples was revealed by RAPD analysis.
| 原文 | 英語 |
|---|---|
| 頁（從 - 到） | 11-14 |
| 頁數 | 4 |
| 期刊 | Botanical Bulletin of Academia Sinica |
| 卷 | 41 |
| 發行號 | 1 |
| 出版狀態 | 已發佈 - 一月 2000 |
指紋
ASJC Scopus subject areas
- Plant Science
引用此文
RAPD analysis of Lycium barbarum medicine in Taiwan market. / Cheng, Kur Ta; Chang, Hsien Chang; Huang, Her; Lin, Cher Tong.
於: Botanical Bulletin of Academia Sinica, 卷 41, 編號 1, 01.2000, p. 11-14.研究成果: 雜誌貢獻 › 文章
}
TY - JOUR
T1 - RAPD analysis of Lycium barbarum medicine in Taiwan market
AU - Cheng, Kur Ta
AU - Chang, Hsien Chang
AU - Huang, Her
AU - Lin, Cher Tong
PY - 2000/1
Y1 - 2000/1
N2 - In this study, we investigated Lycium barbarum, a Chinese medicine sold on the Taiwan market, using RAPD analysis. Sixty oligonucleotide primers were used to screen twenty randomly selected samples in the analysis. Total DNA extracted from the fruit of the medicine material was used as template in the PCR reaction. Four primers: OPD-15, OPG-15, OPT-12 and OPT-17, showed distinct polymorphic patterns, but others exhibited profiles nearly identical to the other samples used in the study. We found that only two RAPD fingerprinting types of these primers were outlined from twenty collected Lycium samples. Fifteen samples showed the first type of profiles while only five samples resulted of the second type. A low genetic diversity among the Lycium barbarum samples was revealed by RAPD analysis.
AB - In this study, we investigated Lycium barbarum, a Chinese medicine sold on the Taiwan market, using RAPD analysis. Sixty oligonucleotide primers were used to screen twenty randomly selected samples in the analysis. Total DNA extracted from the fruit of the medicine material was used as template in the PCR reaction. Four primers: OPD-15, OPG-15, OPT-12 and OPT-17, showed distinct polymorphic patterns, but others exhibited profiles nearly identical to the other samples used in the study. We found that only two RAPD fingerprinting types of these primers were outlined from twenty collected Lycium samples. Fifteen samples showed the first type of profiles while only five samples resulted of the second type. A low genetic diversity among the Lycium barbarum samples was revealed by RAPD analysis.
KW - Chinese medicine
KW - DNA
KW - Lycium barbarum
KW - RAPD
UR - http://www.scopus.com/inward/record.url?scp=0033962464&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033962464&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0033962464
VL - 41
SP - 11
EP - 14
JO - Botanical Studies
JF - Botanical Studies
SN - 1817-406X
IS - 1
ER -