摘要
| 原文 | 英語 |
|---|---|
| 頁（從 - 到） | 841-852 |
| 頁數 | 12 |
| 期刊 | International Journal of Radiation Biology |
| 卷 | 90 |
| 發行號 | 10 |
| DOIs | |
| 出版狀態 | 已發佈 - 十月 1 2014 |
指紋
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging
- Radiological and Ultrasound Technology
- Medicine(all)
引用此文
Radioprotective effects of Antrodia cinnamomea are enhanced on immune cells and inhibited on cancer cells. / Cheng, Po Ching; Huang, Chun Chih; Chiang, Ping Fang; Lin, Ching Nan; Li, Li Li; Lee, Te Wei; Lin, Bin; Chen, I. Chen; Chang, Kang Wei; Fan, Chia Kwung; Luo, Tsai Yueh.
於: International Journal of Radiation Biology, 卷 90, 編號 10, 01.10.2014, p. 841-852.研究成果: 雜誌貢獻 › 文章
}
TY - JOUR
T1 - Radioprotective effects of Antrodia cinnamomea are enhanced on immune cells and inhibited on cancer cells
AU - Cheng, Po Ching
AU - Huang, Chun Chih
AU - Chiang, Ping Fang
AU - Lin, Ching Nan
AU - Li, Li Li
AU - Lee, Te Wei
AU - Lin, Bin
AU - Chen, I. Chen
AU - Chang, Kang Wei
AU - Fan, Chia Kwung
AU - Luo, Tsai Yueh
PY - 2014/10/1
Y1 - 2014/10/1
N2 - Purpose: The radioprotective effects of Antrodia cinnamomea (AC) were investigated for understanding the potential usefulness of AC as an adjunct treatment for reducing radiation side-effects. Materials and methods: In this study, we determined the ability of AC extracts (AC539) to reduce radiation side-effects by analyzing cellular viability in normal mouse spleen immune cells and human cancer cells with different radiosensitivity. We further detected the effect of AC on radiation-induced changes in cytokine- and inflammatory-related gene expressions. Furthermore, apoptosis assay was performed to determine whether AC could inhibit radiation-induced cytotoxicity. Results: We found that an AC dose of 100-150 μg/ml in a time-dependent manner was the most effective in blocking radiation-induced cytotoxicity, in vitro. Radiation-induced cytotoxicity was inhibited in spleen immune cells by 37-56%; however, pretreatment of human colorectal cancer cell line HT-29 with AC did not have any effect on radiation-induced cytotoxicity, while pretreatment of radiosensitive human breast cancer cell lines BT-474 with AC caused a moderate enhancement of radiation-induced damage. Furthermore, AC pretreatment differentially regulated the mRNA expression of several important immunomodulatory genes in response to irradiation in normal and cancer cells. Conclusions: Our data indicate that AC may inhibit important immunoregulatory signaling which could be vital in the avoidance of an over-activated cytotoxic and inflammatory response of the immune system caused by radiation-induced tissue damage. Additionally, AC does not provide a radioprotective effect to tumor cells but instead enhances radiation-induced inflammation and cytotoxicity in cancer.
AB - Purpose: The radioprotective effects of Antrodia cinnamomea (AC) were investigated for understanding the potential usefulness of AC as an adjunct treatment for reducing radiation side-effects. Materials and methods: In this study, we determined the ability of AC extracts (AC539) to reduce radiation side-effects by analyzing cellular viability in normal mouse spleen immune cells and human cancer cells with different radiosensitivity. We further detected the effect of AC on radiation-induced changes in cytokine- and inflammatory-related gene expressions. Furthermore, apoptosis assay was performed to determine whether AC could inhibit radiation-induced cytotoxicity. Results: We found that an AC dose of 100-150 μg/ml in a time-dependent manner was the most effective in blocking radiation-induced cytotoxicity, in vitro. Radiation-induced cytotoxicity was inhibited in spleen immune cells by 37-56%; however, pretreatment of human colorectal cancer cell line HT-29 with AC did not have any effect on radiation-induced cytotoxicity, while pretreatment of radiosensitive human breast cancer cell lines BT-474 with AC caused a moderate enhancement of radiation-induced damage. Furthermore, AC pretreatment differentially regulated the mRNA expression of several important immunomodulatory genes in response to irradiation in normal and cancer cells. Conclusions: Our data indicate that AC may inhibit important immunoregulatory signaling which could be vital in the avoidance of an over-activated cytotoxic and inflammatory response of the immune system caused by radiation-induced tissue damage. Additionally, AC does not provide a radioprotective effect to tumor cells but instead enhances radiation-induced inflammation and cytotoxicity in cancer.
KW - Antrodia cinnamomea
KW - apoptosis
KW - cancer
KW - radiation side-effects
KW - radioprotection
UR - http://www.scopus.com/inward/record.url?scp=84904794221&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84904794221&partnerID=8YFLogxK
U2 - 10.3109/09553002.2014.911989
DO - 10.3109/09553002.2014.911989
M3 - Article
C2 - 24708166
AN - SCOPUS:84904794221
VL - 90
SP - 841
EP - 852
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
SN - 0955-3002
IS - 10
ER -