Quercetin induced cell apoptosis and altered gene expression in AGS human gastric cancer cells

Hung Sheng Shang, Hsu Feng Lu, Ching Hsiao Lee, Han Sun Chiang, Yung Lin Chu, Ann Chen, Yuh Feng Lin, Jing Gung Chung

研究成果: 雜誌貢獻文章

4 引文 (Scopus)

摘要

Quercetin is one of the natural components from natural plant and it induces cell apoptosis in many human cancer cell lines. However, no available reports show that quercetin induces apoptosis and altered associated gene expressions in human gastric cancer cells, thus, we investigated the effect of quercetin on the apoptotic cell death and associated gene expression in human gastric cancer AGS cells. Results indicated that quercetin induced cell morphological changes and reduced total viability via apoptotic cell death in AGS cells. Furthermore, results from flow cytometric assay indicated that quercetin increased reactive oxygen species (ROS) production, decreased the levels of mitochondrial membrane potential (ΔΨm), and increased the apoptotic cell number in AGS cells. Results from western blotting showed that quercetin decreased anti-apoptotic protein of Mcl-1, Bcl-2, and Bcl-x but increased pro-apoptotic protein of Bad, Bax, and Bid. Furthermore, quercetin increased the gene expressions of TNFRSF10D (Tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain), TP53INP1 (tumor protein p53 inducible nuclear protein 1), and JUNB (jun B proto-oncogene) but decreased the gene expression of VEGFB (vascular endothelial growth factor B), CDK10 (cyclin-dependent kinase 10), and KDELC2 (KDEL [Lys-Asp-Glu-Leu] containing 2) that are associated with apoptosis pathways. Thus, those findings may offer more information regarding the molecular, gene expression, and signaling pathway for quercetin induced apoptotic cell death in human gastric cancer cells.
原文英語
頁(從 - 到)1168-1181
頁數14
期刊Environmental Toxicology
33
發行號11
DOIs
出版狀態已發佈 - 十一月 1 2018

指紋

Quercetin
apoptosis
Gene expression
Stomach Neoplasms
gene expression
cancer
Cells
Apoptosis
Gene Expression
protein
Cell death
tumor
Apoptosis Regulatory Proteins
Cell Death
lysyl-aspartyl-glutamyl-leucine
Vascular Endothelial Growth Factor B
jun Genes
TNF-Related Apoptosis-Inducing Ligand Receptors
Plant Structures
viability

Keywords

  • apoptosis
  • cDNA
  • gene expression
  • human gastric cancer cells
  • quercetin

ASJC Scopus subject areas

  • Toxicology
  • Management, Monitoring, Policy and Law
  • Health, Toxicology and Mutagenesis

引用此文

Shang, H. S., Lu, H. F., Lee, C. H., Chiang, H. S., Chu, Y. L., Chen, A., ... Chung, J. G. (2018). Quercetin induced cell apoptosis and altered gene expression in AGS human gastric cancer cells. Environmental Toxicology, 33(11), 1168-1181. https://doi.org/10.1002/tox.22623

Quercetin induced cell apoptosis and altered gene expression in AGS human gastric cancer cells. / Shang, Hung Sheng; Lu, Hsu Feng; Lee, Ching Hsiao; Chiang, Han Sun; Chu, Yung Lin; Chen, Ann; Lin, Yuh Feng; Chung, Jing Gung.

於: Environmental Toxicology, 卷 33, 編號 11, 01.11.2018, p. 1168-1181.

研究成果: 雜誌貢獻文章

Shang, HS, Lu, HF, Lee, CH, Chiang, HS, Chu, YL, Chen, A, Lin, YF & Chung, JG 2018, 'Quercetin induced cell apoptosis and altered gene expression in AGS human gastric cancer cells', Environmental Toxicology, 卷 33, 編號 11, 頁 1168-1181. https://doi.org/10.1002/tox.22623
Shang, Hung Sheng ; Lu, Hsu Feng ; Lee, Ching Hsiao ; Chiang, Han Sun ; Chu, Yung Lin ; Chen, Ann ; Lin, Yuh Feng ; Chung, Jing Gung. / Quercetin induced cell apoptosis and altered gene expression in AGS human gastric cancer cells. 於: Environmental Toxicology. 2018 ; 卷 33, 編號 11. 頁 1168-1181.
@article{5c1f7ed6d31d4e95936bfd148a3c84ed,
title = "Quercetin induced cell apoptosis and altered gene expression in AGS human gastric cancer cells",
abstract = "Quercetin is one of the natural components from natural plant and it induces cell apoptosis in many human cancer cell lines. However, no available reports show that quercetin induces apoptosis and altered associated gene expressions in human gastric cancer cells, thus, we investigated the effect of quercetin on the apoptotic cell death and associated gene expression in human gastric cancer AGS cells. Results indicated that quercetin induced cell morphological changes and reduced total viability via apoptotic cell death in AGS cells. Furthermore, results from flow cytometric assay indicated that quercetin increased reactive oxygen species (ROS) production, decreased the levels of mitochondrial membrane potential (ΔΨm), and increased the apoptotic cell number in AGS cells. Results from western blotting showed that quercetin decreased anti-apoptotic protein of Mcl-1, Bcl-2, and Bcl-x but increased pro-apoptotic protein of Bad, Bax, and Bid. Furthermore, quercetin increased the gene expressions of TNFRSF10D (Tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain), TP53INP1 (tumor protein p53 inducible nuclear protein 1), and JUNB (jun B proto-oncogene) but decreased the gene expression of VEGFB (vascular endothelial growth factor B), CDK10 (cyclin-dependent kinase 10), and KDELC2 (KDEL [Lys-Asp-Glu-Leu] containing 2) that are associated with apoptosis pathways. Thus, those findings may offer more information regarding the molecular, gene expression, and signaling pathway for quercetin induced apoptotic cell death in human gastric cancer cells.",
keywords = "apoptosis, cDNA, gene expression, human gastric cancer cells, quercetin, apoptosis, cDNA, gene expression, human gastric cancer cells, quercetin",
author = "Shang, {Hung Sheng} and Lu, {Hsu Feng} and Lee, {Ching Hsiao} and Chiang, {Han Sun} and Chu, {Yung Lin} and Ann Chen and Lin, {Yuh Feng} and Chung, {Jing Gung}",
year = "2018",
month = "11",
day = "1",
doi = "10.1002/tox.22623",
language = "English",
volume = "33",
pages = "1168--1181",
journal = "Environmental Toxicology",
issn = "1520-4081",
publisher = "John Wiley and Sons Inc.",
number = "11",

}

TY - JOUR

T1 - Quercetin induced cell apoptosis and altered gene expression in AGS human gastric cancer cells

AU - Shang, Hung Sheng

AU - Lu, Hsu Feng

AU - Lee, Ching Hsiao

AU - Chiang, Han Sun

AU - Chu, Yung Lin

AU - Chen, Ann

AU - Lin, Yuh Feng

AU - Chung, Jing Gung

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Quercetin is one of the natural components from natural plant and it induces cell apoptosis in many human cancer cell lines. However, no available reports show that quercetin induces apoptosis and altered associated gene expressions in human gastric cancer cells, thus, we investigated the effect of quercetin on the apoptotic cell death and associated gene expression in human gastric cancer AGS cells. Results indicated that quercetin induced cell morphological changes and reduced total viability via apoptotic cell death in AGS cells. Furthermore, results from flow cytometric assay indicated that quercetin increased reactive oxygen species (ROS) production, decreased the levels of mitochondrial membrane potential (ΔΨm), and increased the apoptotic cell number in AGS cells. Results from western blotting showed that quercetin decreased anti-apoptotic protein of Mcl-1, Bcl-2, and Bcl-x but increased pro-apoptotic protein of Bad, Bax, and Bid. Furthermore, quercetin increased the gene expressions of TNFRSF10D (Tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain), TP53INP1 (tumor protein p53 inducible nuclear protein 1), and JUNB (jun B proto-oncogene) but decreased the gene expression of VEGFB (vascular endothelial growth factor B), CDK10 (cyclin-dependent kinase 10), and KDELC2 (KDEL [Lys-Asp-Glu-Leu] containing 2) that are associated with apoptosis pathways. Thus, those findings may offer more information regarding the molecular, gene expression, and signaling pathway for quercetin induced apoptotic cell death in human gastric cancer cells.

AB - Quercetin is one of the natural components from natural plant and it induces cell apoptosis in many human cancer cell lines. However, no available reports show that quercetin induces apoptosis and altered associated gene expressions in human gastric cancer cells, thus, we investigated the effect of quercetin on the apoptotic cell death and associated gene expression in human gastric cancer AGS cells. Results indicated that quercetin induced cell morphological changes and reduced total viability via apoptotic cell death in AGS cells. Furthermore, results from flow cytometric assay indicated that quercetin increased reactive oxygen species (ROS) production, decreased the levels of mitochondrial membrane potential (ΔΨm), and increased the apoptotic cell number in AGS cells. Results from western blotting showed that quercetin decreased anti-apoptotic protein of Mcl-1, Bcl-2, and Bcl-x but increased pro-apoptotic protein of Bad, Bax, and Bid. Furthermore, quercetin increased the gene expressions of TNFRSF10D (Tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain), TP53INP1 (tumor protein p53 inducible nuclear protein 1), and JUNB (jun B proto-oncogene) but decreased the gene expression of VEGFB (vascular endothelial growth factor B), CDK10 (cyclin-dependent kinase 10), and KDELC2 (KDEL [Lys-Asp-Glu-Leu] containing 2) that are associated with apoptosis pathways. Thus, those findings may offer more information regarding the molecular, gene expression, and signaling pathway for quercetin induced apoptotic cell death in human gastric cancer cells.

KW - apoptosis

KW - cDNA

KW - gene expression

KW - human gastric cancer cells

KW - quercetin

KW - apoptosis

KW - cDNA

KW - gene expression

KW - human gastric cancer cells

KW - quercetin

UR - http://www.scopus.com/inward/record.url?scp=85052820492&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85052820492&partnerID=8YFLogxK

U2 - 10.1002/tox.22623

DO - 10.1002/tox.22623

M3 - Article

C2 - 30152185

AN - SCOPUS:85052820492

VL - 33

SP - 1168

EP - 1181

JO - Environmental Toxicology

JF - Environmental Toxicology

SN - 1520-4081

IS - 11

ER -