Quercetin induced cell apoptosis and altered gene expression in AGS human gastric cancer cells

Hung Sheng Shang, Hsu Feng Lu, Ching Hsiao Lee, Han Sun Chiang, Yung Lin Chu, Ann Chen, Yuh Feng Lin, Jing Gung Chung

研究成果: 雜誌貢獻文章

7 引文 斯高帕斯(Scopus)

摘要

Quercetin is one of the natural components from natural plant and it induces cell apoptosis in many human cancer cell lines. However, no available reports show that quercetin induces apoptosis and altered associated gene expressions in human gastric cancer cells, thus, we investigated the effect of quercetin on the apoptotic cell death and associated gene expression in human gastric cancer AGS cells. Results indicated that quercetin induced cell morphological changes and reduced total viability via apoptotic cell death in AGS cells. Furthermore, results from flow cytometric assay indicated that quercetin increased reactive oxygen species (ROS) production, decreased the levels of mitochondrial membrane potential (ΔΨm), and increased the apoptotic cell number in AGS cells. Results from western blotting showed that quercetin decreased anti-apoptotic protein of Mcl-1, Bcl-2, and Bcl-x but increased pro-apoptotic protein of Bad, Bax, and Bid. Furthermore, quercetin increased the gene expressions of TNFRSF10D (Tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain), TP53INP1 (tumor protein p53 inducible nuclear protein 1), and JUNB (jun B proto-oncogene) but decreased the gene expression of VEGFB (vascular endothelial growth factor B), CDK10 (cyclin-dependent kinase 10), and KDELC2 (KDEL [Lys-Asp-Glu-Leu] containing 2) that are associated with apoptosis pathways. Thus, those findings may offer more information regarding the molecular, gene expression, and signaling pathway for quercetin induced apoptotic cell death in human gastric cancer cells.
原文英語
頁(從 - 到)1168-1181
頁數14
期刊Environmental Toxicology
33
發行號11
DOIs
出版狀態已發佈 - 十一月 1 2018

    指紋

Keywords

  • apoptosis
  • cDNA
  • gene expression
  • human gastric cancer cells
  • quercetin

ASJC Scopus subject areas

  • Toxicology
  • Management, Monitoring, Policy and Law
  • Health, Toxicology and Mutagenesis

引用此

Shang, H. S., Lu, H. F., Lee, C. H., Chiang, H. S., Chu, Y. L., Chen, A., Lin, Y. F., & Chung, J. G. (2018). Quercetin induced cell apoptosis and altered gene expression in AGS human gastric cancer cells. Environmental Toxicology, 33(11), 1168-1181. https://doi.org/10.1002/tox.22623