Quercetin in elimination of tumor initiating stem-like and mesenchymal transformation property in head and neck cancer

Wen Wei Chang, Fang Wei Hu, Cheng Chia Yu, Hsiu Huan Wang, Hsiang Pu Feng, Chih Lan, Lo Lin Tsai, Yu Chao Chang

研究成果: 雜誌貢獻文章

32 引文 (Scopus)

摘要

Background Previously, we enriched a subpopulation of head and neck cancer-derived tumor initiating cells (HNC-TICs) presented high tumorigenic, chemo-radioresistant, and coupled with epithelial-mesenchymal transition (EMT) properties. The purpose of this study was to investigate the therapeutic effect and molecular mechanisms of quercetin on HNC-TICs. Method ALDH1 activity of head and neck cancer cells with quercetin treatment was assessed by the Aldefluor assay flow cytometry analysis. Self-renewal, invasiveness, and EMT capability of HNC-TICs with different doses of quercetin was presented. Results We first observed that the treatment of quercetin significantly downregulated the ALDH1 activity of head and neck cancer cells in a dose-dependent manner (p <.05). Moreover, quercetin reduced self-renewal property and stemness signatures expression in head and neck cancer-derived sphere cells. The migration ability of head and neck cancer-derived sphere cells was lessened under quercetin treatment partially due to the decreased productions of Twist, N-cadherin, and vimentin. Conclusion Quercetin suppressing HNC-TICs characteristics may therefore be valuable therapeutics clinically in combination with standard treatment modalities.
原文英語
頁(從 - 到)413-419
頁數7
期刊Head and Neck
35
發行號3
DOIs
出版狀態已發佈 - 三月 2013
對外發佈Yes

指紋

Quercetin
Head and Neck Neoplasms
Neoplastic Stem Cells
Neoplasms
Epithelial-Mesenchymal Transition
Therapeutic Uses
Vimentin
Cadherins
Flow Cytometry
Down-Regulation

ASJC Scopus subject areas

  • Otorhinolaryngology

引用此文

Chang, W. W., Hu, F. W., Yu, C. C., Wang, H. H., Feng, H. P., Lan, C., ... Chang, Y. C. (2013). Quercetin in elimination of tumor initiating stem-like and mesenchymal transformation property in head and neck cancer. Head and Neck, 35(3), 413-419. https://doi.org/10.1002/hed.22982

Quercetin in elimination of tumor initiating stem-like and mesenchymal transformation property in head and neck cancer. / Chang, Wen Wei; Hu, Fang Wei; Yu, Cheng Chia; Wang, Hsiu Huan; Feng, Hsiang Pu; Lan, Chih; Tsai, Lo Lin; Chang, Yu Chao.

於: Head and Neck, 卷 35, 編號 3, 03.2013, p. 413-419.

研究成果: 雜誌貢獻文章

Chang, Wen Wei ; Hu, Fang Wei ; Yu, Cheng Chia ; Wang, Hsiu Huan ; Feng, Hsiang Pu ; Lan, Chih ; Tsai, Lo Lin ; Chang, Yu Chao. / Quercetin in elimination of tumor initiating stem-like and mesenchymal transformation property in head and neck cancer. 於: Head and Neck. 2013 ; 卷 35, 編號 3. 頁 413-419.
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AB - Background Previously, we enriched a subpopulation of head and neck cancer-derived tumor initiating cells (HNC-TICs) presented high tumorigenic, chemo-radioresistant, and coupled with epithelial-mesenchymal transition (EMT) properties. The purpose of this study was to investigate the therapeutic effect and molecular mechanisms of quercetin on HNC-TICs. Method ALDH1 activity of head and neck cancer cells with quercetin treatment was assessed by the Aldefluor assay flow cytometry analysis. Self-renewal, invasiveness, and EMT capability of HNC-TICs with different doses of quercetin was presented. Results We first observed that the treatment of quercetin significantly downregulated the ALDH1 activity of head and neck cancer cells in a dose-dependent manner (p <.05). Moreover, quercetin reduced self-renewal property and stemness signatures expression in head and neck cancer-derived sphere cells. The migration ability of head and neck cancer-derived sphere cells was lessened under quercetin treatment partially due to the decreased productions of Twist, N-cadherin, and vimentin. Conclusion Quercetin suppressing HNC-TICs characteristics may therefore be valuable therapeutics clinically in combination with standard treatment modalities.

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