Quantitative DNA methylation analysis detects cervical intraepithelial neoplasms type 3 and worse

Hung Cheng Lai, Ya Wen Lin, Rui Lan Huang, Ming Tzeung Chung, Hui Chen Wang, Yu Ping Liao, Po Hsuan Su, Yung Liang Liu, Mu Hsien Yu

研究成果: 雜誌貢獻文章

69 引文 斯高帕斯(Scopus)

摘要

BACKGROUND: DNA methylation may be used a potential biomarker for detecting cervical cancer. The authors of this report used quantitative methylation analysis of 4 genes in a full spectrum of cervical lesions to test its potential clinical application. METHODS: This hospital-based, retrospective, case-control study was conducted in 185 patients and included patients who had a normal uterine cervix (n = 53), cervical intraepithelial neoplasm type 1 (CIN1) (n = 37), CIN2 (n = 22), CIN3 (n = 24), carcinoma in situ (CIS) (n = 22), squamous cell carcinoma (SCC, n = 20), and adenocarcinoma (AC) (n = 7). Methylation levels of the genes sex-determining region Y, box 1 (SOX1); paired box gene 1 (PAX1); LIM homeobox transcription factor 1a (LMX1A), and NK6 transcription factor-related locus 1 (NKX6-1) were determined by using real-time methylation-specific polymerase chain reaction (PCR) amplification. Cutoff values of the percentage of methylation reference (PMR) for different diagnoses were determined to test the sensitivity and specificity and to generate receiver operating characteristic (ROC) curves. Two-sided Mann-Whitney U tests were used to test differences in PMR between groups. RESULTS: The PMRs of the 4 genes were significantly higher in CIN3 and worse (CIN3+) lesions than the PMRs in specimens of normal cervix and CIN1 or CIN2 (P <.001). ROC curve analysis demonstrated that the sensitivity, specificity, and accuracy for detecting CIN3+ lesions were 0.88, 0.82, and 0.95, respectively, for SOX1; 0.78, 0.91, and 0.89, respectively, for PAX1; 0.77, 0.88, and 0.90, respectively, for LMX1A; and 0.93, 0.97, and 0.97, respectively, for NKX6-1. CONCLUSIONS: The current results indicated that quantitative PCR-based testing for DNA methylation of 4 genes holds great promise for cervical cancer screening and warrants further population-based studies using standardized DNA methylation testing.
原文英語
頁(從 - 到)4266-4274
頁數9
期刊Cancer
116
發行號18
DOIs
出版狀態已發佈 - 九月 15 2010
對外發佈Yes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

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    Lai, H. C., Lin, Y. W., Huang, R. L., Chung, M. T., Wang, H. C., Liao, Y. P., Su, P. H., Liu, Y. L., & Yu, M. H. (2010). Quantitative DNA methylation analysis detects cervical intraepithelial neoplasms type 3 and worse. Cancer, 116(18), 4266-4274. https://doi.org/10.1002/cncr.25252