Quantitative assessment of the kinetics of growth factors release from platelet gel

Chen Y. Su, Ya P. Kuo, Heng Lu Nieh, Yu H. Tseng, Thierry Burnouf

研究成果: 雜誌貢獻文章

65 引文 (Scopus)

摘要

BACKGROUND: The time course of the release of growth factors from platelet (PLT) gels has not been thoroughly studied and should be elucidated for a better standardization of the clinical use of these products. STUDY DESIGN AND METHODS: Release of PLT-derived growth factor-AB (PDGF-AB), transforming growth factor-β1 (TGF-β1), epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), and insulin-like growth factor-1 (IGF-1) was determined 5, 60, 120, and 300 minutes after PLT gel formation. Control experiments where PLT gel was removed and, afterward, exogenous thrombin was added, were also performed. Protein profiles of the PLT concentrates and of the releasates were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). RESULTS: Mean PDGF-AB, TGF-β1, EGF, and VEGF concentration increased to 76, 114, 3.7, and 0.8 ng per mL, respectively, in the presence of the PLT gel, but remained at approximately 28, 30, 0.28, and 0.34 ng per mL, respectively, when the PLT gel was removed after formation. IGF-1 content remained unchanged (approx. 80 ng/mL). SDS-PAGE analysis showed that several PLT proteins disappear during PLT gel formation and that the protein patterns of the releasates were undistinguishable at the different time points. CONCLUSION: There is a gradual and fast release of PDGF-AB, TGF-β1, EGF, and VEGF from PLT gel for at least 60 to 300 minutes after gel formation, whereas the IGF releasate concentration remains unchanged. This study may provide useful information to improve clinical applications of PLT gels and to design improved blood-derived biomaterials with controlled release of growth factors.
原文英語
頁(從 - 到)2414-2420
頁數7
期刊Transfusion
48
發行號11
DOIs
出版狀態已發佈 - 十一月 2008
對外發佈Yes

指紋

Intercellular Signaling Peptides and Proteins
Blood Platelets
Gels
Transforming Growth Factors
Epidermal Growth Factor
Vascular Endothelial Growth Factor A
Somatomedins
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Proteins
Biocompatible Materials
Thrombin

ASJC Scopus subject areas

  • Hematology
  • Immunology
  • Immunology and Allergy

引用此文

Quantitative assessment of the kinetics of growth factors release from platelet gel. / Su, Chen Y.; Kuo, Ya P.; Nieh, Heng Lu; Tseng, Yu H.; Burnouf, Thierry.

於: Transfusion, 卷 48, 編號 11, 11.2008, p. 2414-2420.

研究成果: 雜誌貢獻文章

Su, Chen Y. ; Kuo, Ya P. ; Nieh, Heng Lu ; Tseng, Yu H. ; Burnouf, Thierry. / Quantitative assessment of the kinetics of growth factors release from platelet gel. 於: Transfusion. 2008 ; 卷 48, 編號 11. 頁 2414-2420.
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abstract = "BACKGROUND: The time course of the release of growth factors from platelet (PLT) gels has not been thoroughly studied and should be elucidated for a better standardization of the clinical use of these products. STUDY DESIGN AND METHODS: Release of PLT-derived growth factor-AB (PDGF-AB), transforming growth factor-β1 (TGF-β1), epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), and insulin-like growth factor-1 (IGF-1) was determined 5, 60, 120, and 300 minutes after PLT gel formation. Control experiments where PLT gel was removed and, afterward, exogenous thrombin was added, were also performed. Protein profiles of the PLT concentrates and of the releasates were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). RESULTS: Mean PDGF-AB, TGF-β1, EGF, and VEGF concentration increased to 76, 114, 3.7, and 0.8 ng per mL, respectively, in the presence of the PLT gel, but remained at approximately 28, 30, 0.28, and 0.34 ng per mL, respectively, when the PLT gel was removed after formation. IGF-1 content remained unchanged (approx. 80 ng/mL). SDS-PAGE analysis showed that several PLT proteins disappear during PLT gel formation and that the protein patterns of the releasates were undistinguishable at the different time points. CONCLUSION: There is a gradual and fast release of PDGF-AB, TGF-β1, EGF, and VEGF from PLT gel for at least 60 to 300 minutes after gel formation, whereas the IGF releasate concentration remains unchanged. This study may provide useful information to improve clinical applications of PLT gels and to design improved blood-derived biomaterials with controlled release of growth factors.",
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AU - Su, Chen Y.

AU - Kuo, Ya P.

AU - Nieh, Heng Lu

AU - Tseng, Yu H.

AU - Burnouf, Thierry

PY - 2008/11

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N2 - BACKGROUND: The time course of the release of growth factors from platelet (PLT) gels has not been thoroughly studied and should be elucidated for a better standardization of the clinical use of these products. STUDY DESIGN AND METHODS: Release of PLT-derived growth factor-AB (PDGF-AB), transforming growth factor-β1 (TGF-β1), epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), and insulin-like growth factor-1 (IGF-1) was determined 5, 60, 120, and 300 minutes after PLT gel formation. Control experiments where PLT gel was removed and, afterward, exogenous thrombin was added, were also performed. Protein profiles of the PLT concentrates and of the releasates were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). RESULTS: Mean PDGF-AB, TGF-β1, EGF, and VEGF concentration increased to 76, 114, 3.7, and 0.8 ng per mL, respectively, in the presence of the PLT gel, but remained at approximately 28, 30, 0.28, and 0.34 ng per mL, respectively, when the PLT gel was removed after formation. IGF-1 content remained unchanged (approx. 80 ng/mL). SDS-PAGE analysis showed that several PLT proteins disappear during PLT gel formation and that the protein patterns of the releasates were undistinguishable at the different time points. CONCLUSION: There is a gradual and fast release of PDGF-AB, TGF-β1, EGF, and VEGF from PLT gel for at least 60 to 300 minutes after gel formation, whereas the IGF releasate concentration remains unchanged. This study may provide useful information to improve clinical applications of PLT gels and to design improved blood-derived biomaterials with controlled release of growth factors.

AB - BACKGROUND: The time course of the release of growth factors from platelet (PLT) gels has not been thoroughly studied and should be elucidated for a better standardization of the clinical use of these products. STUDY DESIGN AND METHODS: Release of PLT-derived growth factor-AB (PDGF-AB), transforming growth factor-β1 (TGF-β1), epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), and insulin-like growth factor-1 (IGF-1) was determined 5, 60, 120, and 300 minutes after PLT gel formation. Control experiments where PLT gel was removed and, afterward, exogenous thrombin was added, were also performed. Protein profiles of the PLT concentrates and of the releasates were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). RESULTS: Mean PDGF-AB, TGF-β1, EGF, and VEGF concentration increased to 76, 114, 3.7, and 0.8 ng per mL, respectively, in the presence of the PLT gel, but remained at approximately 28, 30, 0.28, and 0.34 ng per mL, respectively, when the PLT gel was removed after formation. IGF-1 content remained unchanged (approx. 80 ng/mL). SDS-PAGE analysis showed that several PLT proteins disappear during PLT gel formation and that the protein patterns of the releasates were undistinguishable at the different time points. CONCLUSION: There is a gradual and fast release of PDGF-AB, TGF-β1, EGF, and VEGF from PLT gel for at least 60 to 300 minutes after gel formation, whereas the IGF releasate concentration remains unchanged. This study may provide useful information to improve clinical applications of PLT gels and to design improved blood-derived biomaterials with controlled release of growth factors.

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