Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus

Chee Keng Mok; Shih Cheng Chang; Guang Wu Chen; Yu Lun Lo; Shu Jen Chen; Ho Sheng Wu; Ming Tsan Liu; Feng Yee Chang; Tzou Yien Lin; Shin Ru Shih

doi:10.1016/j.jmii.2013.09.010

Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus

Chee Keng Mok, Shih Cheng Chang, Guang Wu Chen, Yu Lun Lo, Shu Jen Chen, Ho Sheng Wu, Ming Tsan Liu, Feng Yee Chang, Tzou Yien Lin, Shin Ru Shih

研究成果: 雜誌貢獻 › 文章 › 同行評審

6 引文斯高帕斯（Scopus）

摘要

Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan's first imported human case and found R and K in quasispecies.

原文	英語
頁（從 - 到）	465-469
頁數	5
期刊	Journal of Microbiology, Immunology and Infection
卷	48
發行號	4
DOIs	https://doi.org/10.1016/j.jmii.2013.09.010
出版狀態	已發佈 - 八月 1 2015
對外發佈	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology and Microbiology(all)
Microbiology (medical)
Infectious Diseases

UN SDG

此研究成果有助於以下永續發展目標

存取文件

10.1016/j.jmii.2013.09.010

其它文件與鏈接

指紋深入研究「Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus」主題。共同形成了獨特的指紋。

引用此

Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus. / Mok, Chee Keng; Chang, Shih Cheng; Chen, Guang Wu; Lo, Yu Lun; Chen, Shu Jen; Wu, Ho Sheng; Liu, Ming Tsan; Chang, Feng Yee; Lin, Tzou Yien; Shih, Shin Ru.

於: Journal of Microbiology, Immunology and Infection, 卷 48, 編號 4, 01.08.2015, p. 465-469.

研究成果: 雜誌貢獻 › 文章 › 同行評審

Mok, Chee Keng ; Chang, Shih Cheng ; Chen, Guang Wu ; Lo, Yu Lun ; Chen, Shu Jen ; Wu, Ho Sheng ; Liu, Ming Tsan ; Chang, Feng Yee ; Lin, Tzou Yien ; Shih, Shin Ru. / Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus. 於: Journal of Microbiology, Immunology and Infection. 2015 ; 卷 48, 編號 4. 頁 465-469.

@article{52247b1ca0ed438fa8361154a7190631,

title = "Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus",

abstract = "Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan's first imported human case and found R and K in quasispecies.",

keywords = "Avian influenza A virus, H7N9, Oseltamivir-resistant marker, Pyrosequencing",

author = "Mok, {Chee Keng} and Chang, {Shih Cheng} and Chen, {Guang Wu} and Lo, {Yu Lun} and Chen, {Shu Jen} and Wu, {Ho Sheng} and Liu, {Ming Tsan} and Chang, {Feng Yee} and Lin, {Tzou Yien} and Shih, {Shin Ru}",

year = "2015",

month = aug,

day = "1",

doi = "10.1016/j.jmii.2013.09.010",

language = "English",

volume = "48",

pages = "465--469",

journal = "Journal of Microbiology, Immunology and Infection",

issn = "0253-2662",

publisher = "Elsevier Taiwan LLC",

number = "4",

}

TY - JOUR

T1 - Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus

AU - Mok, Chee Keng

AU - Chang, Shih Cheng

AU - Chen, Guang Wu

AU - Lo, Yu Lun

AU - Chen, Shu Jen

AU - Wu, Ho Sheng

AU - Liu, Ming Tsan

AU - Chang, Feng Yee

AU - Lin, Tzou Yien

AU - Shih, Shin Ru

PY - 2015/8/1

Y1 - 2015/8/1

N2 - Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan's first imported human case and found R and K in quasispecies.

AB - Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan's first imported human case and found R and K in quasispecies.

KW - Avian influenza A virus

KW - H7N9

KW - Oseltamivir-resistant marker

KW - Pyrosequencing

UR - http://www.scopus.com/inward/record.url?scp=84938286747&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938286747&partnerID=8YFLogxK

U2 - 10.1016/j.jmii.2013.09.010

DO - 10.1016/j.jmii.2013.09.010

M3 - Article

C2 - 24388586

AN - SCOPUS:84938286747

VL - 48

SP - 465

EP - 469

JO - Journal of Microbiology, Immunology and Infection

JF - Journal of Microbiology, Immunology and Infection

SN - 0253-2662

IS - 4

ER -