摘要
Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan's first imported human case and found R and K in quasispecies.
原文 | 英語 |
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頁(從 - 到) | 465-469 |
頁數 | 5 |
期刊 | Journal of Microbiology, Immunology and Infection |
卷 | 48 |
發行號 | 4 |
DOIs | |
出版狀態 | 已發佈 - 八月 1 2015 |
對外發佈 | Yes |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology and Microbiology(all)
- Microbiology (medical)
- Infectious Diseases