Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus

Chee Keng Mok; Shih Cheng Chang; Guang Wu Chen; Yu Lun Lo; Shu Jen Chen; Ho Sheng Wu; Ming Tsan Liu; Feng Yee Chang; Tzou Yien Lin; Shin Ru Shih

doi:10.1016/j.jmii.2013.09.010

Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus

Chee Keng Mok, Shih Cheng Chang, Guang Wu Chen, Yu Lun Lo, Shu Jen Chen, Ho Sheng Wu, Ming Tsan Liu, Feng Yee Chang, Tzou Yien Lin, Shin Ru Shih

研究成果: 雜誌貢獻 › 文章 › 同行評審

6 引文斯高帕斯（Scopus）

摘要

Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan's first imported human case and found R and K in quasispecies.

原文	英語
頁（從 - 到）	465-469
頁數	5
期刊	Journal of Microbiology, Immunology and Infection
卷	48
發行號	4
DOIs	https://doi.org/10.1016/j.jmii.2013.09.010
出版狀態	已發佈 - 八月 1 2015
對外發佈	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology and Microbiology(all)
Microbiology (medical)
Infectious Diseases

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10.1016/j.jmii.2013.09.010

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Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus. / Mok, Chee Keng; Chang, Shih Cheng; Chen, Guang Wu; Lo, Yu Lun; Chen, Shu Jen; Wu, Ho Sheng; Liu, Ming Tsan; Chang, Feng Yee; Lin, Tzou Yien; Shih, Shin Ru.

於: Journal of Microbiology, Immunology and Infection, 卷 48, 編號 4, 01.08.2015, p. 465-469.

研究成果: 雜誌貢獻 › 文章 › 同行評審

Mok, Chee Keng ; Chang, Shih Cheng ; Chen, Guang Wu ; Lo, Yu Lun ; Chen, Shu Jen ; Wu, Ho Sheng ; Liu, Ming Tsan ; Chang, Feng Yee ; Lin, Tzou Yien ; Shih, Shin Ru. / Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus. 於: Journal of Microbiology, Immunology and Infection. 2015 ; 卷 48, 編號 4. 頁 465-469.

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abstract = "Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan's first imported human case and found R and K in quasispecies.",

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AU - Chen, Shu Jen

AU - Wu, Ho Sheng

AU - Liu, Ming Tsan

AU - Chang, Feng Yee

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