Purification of transferrin from Cohn supernatant I using ion-exchange chromatography

Karl B. McCann, Ben Hughes, John Wu, Joseph Bertolini, Peter T. Gomme

研究成果: 雜誌貢獻文章

10 引文 斯高帕斯(Scopus)

摘要

The present paper describes an anion-exchange chromatography method to separate iron-free apo-Tf (apo-transferrin) from albumin and IgG in Cohn supernatant I. The method uses DEAE-fast flow Sepharose chromatography along with optimized protein concentration (5%, w/v) and column operation conditions (40 g/l, conductivity < 1.0 mS/cm) to resolve apo-Tf from IgG and albumin. The single step purifies apo-Tf to > 90% and provides an efficient means to obtain commercial quantities of the protein.
原文英語
頁(從 - 到)211-217
頁數7
期刊Biotechnology and Applied Biochemistry
42
發行號3
DOIs
出版狀態已發佈 - 十二月 1 2005
對外發佈Yes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Molecular Medicine
  • Biomedical Engineering
  • Applied Microbiology and Biotechnology
  • Drug Discovery
  • Process Chemistry and Technology

指紋 深入研究「Purification of transferrin from Cohn supernatant I using ion-exchange chromatography」主題。共同形成了獨特的指紋。

  • 引用此