Purification of transferrin from Cohn supernatant I using ion-exchange chromatography

Karl B. McCann, Ben Hughes, John Wu, Joseph Bertolini, Peter T. Gomme

研究成果: 雜誌貢獻文章

10 引文 (Scopus)

摘要

The present paper describes an anion-exchange chromatography method to separate iron-free apo-Tf (apo-transferrin) from albumin and IgG in Cohn supernatant I. The method uses DEAE-fast flow Sepharose chromatography along with optimized protein concentration (5%, w/v) and column operation conditions (40 g/l, conductivity < 1.0 mS/cm) to resolve apo-Tf from IgG and albumin. The single step purifies apo-Tf to > 90% and provides an efficient means to obtain commercial quantities of the protein.
原文英語
頁(從 - 到)211-217
頁數7
期刊Biotechnology and Applied Biochemistry
42
發行號3
DOIs
出版狀態已發佈 - 十二月 1 2005
對外發佈Yes

指紋

Ion Exchange Chromatography
Transferrin
Chromatography
Purification
Ion exchange
Proteins
Agarose Chromatography
Sepharose
Anions
Albumins
Negative ions
Iron
Immunoglobulin G
2-diethylaminoethanol

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Molecular Medicine
  • Biomedical Engineering
  • Applied Microbiology and Biotechnology
  • Drug Discovery
  • Process Chemistry and Technology

引用此文

Purification of transferrin from Cohn supernatant I using ion-exchange chromatography. / McCann, Karl B.; Hughes, Ben; Wu, John; Bertolini, Joseph; Gomme, Peter T.

於: Biotechnology and Applied Biochemistry, 卷 42, 編號 3, 01.12.2005, p. 211-217.

研究成果: 雜誌貢獻文章

McCann, Karl B. ; Hughes, Ben ; Wu, John ; Bertolini, Joseph ; Gomme, Peter T. / Purification of transferrin from Cohn supernatant I using ion-exchange chromatography. 於: Biotechnology and Applied Biochemistry. 2005 ; 卷 42, 編號 3. 頁 211-217.
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KW - Iron transport

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