Purification of human ceruloplasmin as a by-product of C1-inhibitor

Ferdinand Kouoh Elombo, Miryana Radosevich, Michel Poulle, Jacques Descamps, Sami Chtourou, Thierry Burnouf, Jean Pierre Catteau, Jean Luc Bernier, Nicole Cotelle

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4 引文 斯高帕斯(Scopus)

摘要

Human ceruloplasmin (Cp) has been purified from cryoprecipitate-poor plasma as a by-product of the C1-inhibitor production chain. Highly purified Cp was obtained by subsequent ion-exchange chromatography on sulfate-Fractogel EMD and TMAE-Fractogel EMD. Treatments for viral safety included application of the solvent-detergent method and two nanofiltration steps using 35- and 15-nm pore size filters at the end of the process. Overall antigen yield was 95 (±5)%. Purified human ceruloplasmin was studied by electron spin resonance (ESR) to characterize its different types of copper complexes and to check its antioxidant properties. We distinguished three types of complexes: One type-2 Cu(II) with g//=2.25 and A//=180 G and two type-1 Cu(II) exhibiting different narrow hyperfine splitting (A//=72 G and A//=90 G) with close g//(2.20 and 2.21). Purified Cp has a specific activity of 24.5±0.2 mU/mg of proteins. This process provides a method for Cp purification that could be easily integrated into modern plasma fractionation.
原文英語
頁(從 - 到)1406-1409
頁數4
期刊Biological and Pharmaceutical Bulletin
23
發行號12
出版狀態已發佈 - 2000
對外發佈Yes

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

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