Purification of growth hormones by reversed-phase high-performance liquid chromatography

Fu Ming Pan, Wen Chang Chang

研究成果: 雜誌貢獻文章

1 引文 斯高帕斯(Scopus)

摘要

Reversed-phase high-performance liquid chromatography (HPLC) on a column of Radial-Pak C18 cartridge was utilized for the purification of a variety of growth hormone (GH) proteins from mammalian, avian, amphibian and fish pituitary glands. Recovery of GH from pituitary glands of up to 0.43% of total protein was obtained with a high degree of homogeneity as revealed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The HPLC-purified GHs show reactions of identity or near identity by immuno-diffusion studies on agar gel. This method offers a convenient and rapid purification of vertebrate GH on an analytical or preparative scale.
原文英語
頁(從 - 到)326-329
頁數4
期刊Journal of Chromatography B: Biomedical Sciences and Applications
613
發行號2
DOIs
出版狀態已發佈 - 四月 2 1993
對外發佈Yes

ASJC Scopus subject areas

  • Chemistry(all)

指紋 深入研究「Purification of growth hormones by reversed-phase high-performance liquid chromatography」主題。共同形成了獨特的指紋。

  • 引用此