Purification of genomic sized herpesvirus DNA using pulse-field electrophoresis

Robert J. Isfort, Daniel Robinson, Hsing Jien Kung

研究成果: 雜誌貢獻文章同行評審

20 引文 斯高帕斯(Scopus)

摘要

A new method for the separation of genomic sized herpesvirus DNA was developed. This method utilizes the improved separation of extremely high molecular weight DNA molecules by pulse-field electrophoresis. This method has allowed us to separate and purify herpesvirus DNA (> 100 kbp) free of cellular DNA, directly from infected cells. In the case of cell associated herpesviruses (such as Marek's disease herpesvirus), this method is more efficient than present isolation procedures. The DNA obtained is able to be restriction enzyme digested and used in cloning experiments.
原文英語
頁(從 - 到)311-317
頁數7
期刊Journal of Virological Methods
27
發行號3
DOIs
出版狀態已發佈 - 1月 1 1990
對外發佈

ASJC Scopus subject areas

  • 病毒學

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