Purification and properties of fatty acid esterases from yam (Dioscorea batatas Decne) tuber

Wen C. Hou, Hsien Jung Chen, Chung Fang Chang, Yaw Huei Lin

研究成果: 雜誌貢獻文章同行評審

9 引文 斯高帕斯(Scopus)

摘要

Using β-naphthyl myristate (C14 fatty acid ester) as a screening substrate, we purified fatty acid esterases (FAEs) from yam (Dioscorea batatas Decne) tuber. Two FAE fractions (named FAE I and FAE II) were obtained after DE-52 ion exchange chromatography and Sephadex G-75 gel filtration purification steps, and then further purified by Con A Sepharose 4B affinity chromatography. FAE I and II fractions contained the same three protein bands about 50-64 kDa corresponding to esterase activity bands on SDS-PAGE gels. Among β-naphthyl esters determined at pH 4.0, 5.0 and 6.0 the best substrate for both FAE fractions was a C10-containing one with a maximum pH at 5.0. The K(m) and V(max) for β-naphthyl caprate (C10 fatty acid ester) of FAE I and II at 37°C, pH 5.0 were 0.338 and 0.959 mM; 0.405 and 0.585 nmole β-naphthol/min μg protein, respectively. FAE activity was stable below 50°C and lost completely above 65°C.

原文英語
頁(從 - 到)305-310
頁數6
期刊Botanical Bulletin of Academia Sinica
40
發行號4
出版狀態已發佈 - 10月 1999
對外發佈

ASJC Scopus subject areas

  • 植物科學

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