Pterostilbene, a bioactive component of blueberries, suppresses the generation of breast cancer stem cells within tumor microenvironment and metastasis via modulating NF-κB/microRNA 448 circuit

Ka Kit Mak, Alexander T H Wu, Wei Hwa Lee, Tung Cheng Chang, Jeng Fong Chiou, Liang Shun Wang, Chih Hsiung Wu, Chi Ying F Huang, Yi Shing Shieh, Tsu Yi Chao, Chi Tang Ho, Gow Chin Yen, Chi-Tai Yeh

研究成果: 雜誌貢獻文章

57 引文 斯高帕斯(Scopus)

摘要

Scope: Tumor-associated macrophages (TAMs) have been shown to promote metastasis and malignancy. Pterostilbene, a natural stilbene isolated from blueberries, has been suggested for anti-cancer effects. Here, we explored the potential cancer stem cells (CSCs)/TAM modulating effects of pterostilbene in breast cancer. Methods and results: Using flowcytometric and Boyden chamber assay, we showed MCF7 and MDA-MB-231 cells cocultured with M2 TAMs exhibited increased percentage of CD44+/CD24- CSC population and migratory/invasive abilities. RT-PCR results showed that CD44+/CD24- cells expressed an increased level of HIF-1α, β-catenin, Twist1, and NF-κB and enhanced tumor sphere forming ability. Additionally, pterostilbene treatment dose dependently overcame M2 TAM-induced enrichment of CSCs and metastatic potential of breast cancer cells. Mechanistically, pterostilbene suppressed NFκB, Twist1, vimentin, and increased E-cadherin expression. Using siRNA technique, we demonstrated that pterostilbene-mediated NFκB downregulation was correlated to an increased amount of microRNA 448. Finally, pterostilbene-mediated suppression in tumorigenesis and metastasis was validated by noninvasive bioluminescence in mice bearing M2 TAM cocultured MDA-MB-231 tumor.
原文英語
頁(從 - 到)1123-1134
頁數12
期刊Molecular Nutrition and Food Research
57
發行號7
DOIs
出版狀態已發佈 - 七月 2013

ASJC Scopus subject areas

  • Food Science
  • Biotechnology

指紋 深入研究「Pterostilbene, a bioactive component of blueberries, suppresses the generation of breast cancer stem cells within tumor microenvironment and metastasis via modulating NF-κB/microRNA 448 circuit」主題。共同形成了獨特的指紋。

  • 引用此