Proteomics characterization of cytoplasmic and lipid-associated membrane proteins of human pathogen mycoplasma fermentans m64

Yi Chang Liu, I. Hsuan Lin, Wei Jen Chung, Wensi S. Hu, Wailap Victor Ng, Chi Yu Lu, Tsung Yen Huang, Hung Wei Shu, Kwang Jen Hsiao, Shih Feng Tsai, Chuan Hsiung Chang, Chao Hsiung Lin

研究成果: 雜誌貢獻文章

11 引文 (Scopus)

摘要

Mycoplasma fermentans is a potent human pathogen which has been implicated in several diseases. Notably, its lipid-associated membrane proteins (LAMPs) play a role in immunomodulation and development of infection-associated inflammatory diseases. However, the systematic protein identification of pathogenic M. fermentans has not been reported. From our recent sequencing results of M. fermentans M64 isolated from human respiratory tract, its genome is around 1.1 Mb and encodes 1050 predicted protein-coding genes. In the present study, soluble proteome of M. fermentans was resolved and analyzed using two-dimensional gel electrophoresis. In addition, Triton X-114 extraction was carried out to enrich amphiphilic proteins including putative lipoproteins and membrane proteins. Subsequent mass spectrometric analyses of these proteins had identified a total of 181 M. fermentans ORFs. Further bioinformatics analysis of these ORFs encoding proteins with known or so far unknown orthologues among bacteria revealed that a total of 131 proteins are homologous to known proteins, 11 proteins are conserved hypothetical proteins, and the remaining 39 proteins are likely M. fermentans-specific proteins. Moreover, Triton X-114-enriched fraction was shown to activate NF-kB activity of raw264.7 macrophage and a total of 21 lipoproteins with predicted signal peptide were identified therefrom. Together, our work provides the first proteome reference map of M. fermentans as well as several putative virulence-associated proteins as diagnostic markers or vaccine candidates for further functional study of this human pathogen.
原文英語
文章編號e35304
期刊PLoS One
7
發行號4
DOIs
出版狀態已發佈 - 四月 20 2012
對外發佈Yes

指紋

Mycoplasma fermentans
Pathogens
membrane proteins
Proteomics
proteomics
Membrane Proteins
Lipids
pathogens
lipids
Proteins
proteins
Proteome
proteome
lipoproteins
Open Reading Frames
Lipoproteins
open reading frames
Marker Vaccines
Genes
transcription factor NF-kappa B

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

引用此文

Proteomics characterization of cytoplasmic and lipid-associated membrane proteins of human pathogen mycoplasma fermentans m64. / Liu, Yi Chang; Lin, I. Hsuan; Chung, Wei Jen; Hu, Wensi S.; Ng, Wailap Victor; Lu, Chi Yu; Huang, Tsung Yen; Shu, Hung Wei; Hsiao, Kwang Jen; Tsai, Shih Feng; Chang, Chuan Hsiung; Lin, Chao Hsiung.

於: PLoS One, 卷 7, 編號 4, e35304, 20.04.2012.

研究成果: 雜誌貢獻文章

Liu, YC, Lin, IH, Chung, WJ, Hu, WS, Ng, WV, Lu, CY, Huang, TY, Shu, HW, Hsiao, KJ, Tsai, SF, Chang, CH & Lin, CH 2012, 'Proteomics characterization of cytoplasmic and lipid-associated membrane proteins of human pathogen mycoplasma fermentans m64', PLoS One, 卷 7, 編號 4, e35304. https://doi.org/10.1371/journal.pone.0035304
Liu, Yi Chang ; Lin, I. Hsuan ; Chung, Wei Jen ; Hu, Wensi S. ; Ng, Wailap Victor ; Lu, Chi Yu ; Huang, Tsung Yen ; Shu, Hung Wei ; Hsiao, Kwang Jen ; Tsai, Shih Feng ; Chang, Chuan Hsiung ; Lin, Chao Hsiung. / Proteomics characterization of cytoplasmic and lipid-associated membrane proteins of human pathogen mycoplasma fermentans m64. 於: PLoS One. 2012 ; 卷 7, 編號 4.
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