Proteomic analysis of differentially expressed proteins in the lymphoid organ of Vibrio harveyi-infected Penaeus monodon

Vorrapon Chaikeeratisak, Kunlaya Somboonwiwat, Hao Ching Wang, Chu Fang Lo, Anchalee Tassanakajon

研究成果: 雜誌貢獻文章

16 引文 斯高帕斯(Scopus)

摘要

The protein expression profiles of the lymphoid organ, taken from mock and systemic Vibrio harveyiinfected Penaeus monodon at 6 and 48 h post infection, were revealed. The considerable changes in the expression level of several proteins were observed between the mock and V. harveyi-infected shrimps. From 30 analyzed protein spots with 27 differentially expressed, 21 were known proteins with the most common of these being cytoskeleton proteins (33%) which were all down-regulated upon systemic bacterial infection. Other six proteins including four proteins that are involved in the shrimp immunity (alpha-2- macroglobulin, transglutaminase, heat shock protein 1 and hemocyanin subunit Y), and two proteins that are involved in metabolism (triosephosphate isomerase) and cell signaling (14-3-3 like protein), displayed significantly decreased expression levels. There was, however, an increase in the expression level of the ATP synthase beta subunit, a protein involved in energy balance. Transcription levels of ATP synthase beta subunit and 14-3-3 like protein were up- and down-regulated, respectively, in accord with the observed protein expression levels, but the alpha-2-macroglobulin transcript levels were significantly increased in contrast to the decreased protein expression levels. Interestingly, partial gene silencing of ATP synthase beta subunit revealed a high cumulative mortality of the knockdown shrimps (73.3%) and a dramatic reduction of the total hemocyte numbers in the survival shrimps. These altered proteins are likely to play essential roles in shrimp defense against the pathogenic bacterium V. harveyi.
原文英語
頁(從 - 到)6367-6377
頁數11
期刊Molecular Biology Reports
39
發行號5
DOIs
出版狀態已發佈 - 五月 2012
對外發佈Yes

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ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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