Protective effects of solvent extracts from Taiwanese Agrocybe cylindracea strain B against DNA damage induced by environmental mutagens

Shwu Yuann Lin, Lien H. Wang, Huei Lee, Ben T. Chiang, Shun J. Tsai, Meei Y. Lin

研究成果: 雜誌貢獻文章同行評審

2 引文 斯高帕斯(Scopus)

摘要

Antioxidant activity of a water extract from Agrocybe cylindracea strain B (ACB) against iron-mediated lipid peroxidation has been demonstrated. In addition, the protective effect of water extracts from ACB (WAC) on hydroxyl radical-mediated DNA strand breaks was better than that of the shiitake mushroom (Lentinula edodes). Therefore, we decided to investigate whether different solvent extracts from ACB (ACES) protect DNA against oxidative stress induced by environmental mutagens, such as cooking oil fumes (COF) and benzo[a]pyrene (BaP). Oxidative DNA damage and intercellular DNA migration (tail length) were quantified by determining the decrease of extracellular supercoiled (SC) plasmid DNA and by using the 'comet assay' in the human adenocarcinoma CL-3 cell line, respectively. IC50 values of water, boiled water, methanol and acetone extracts from ACB were 108.97, 87.21, 970.52 and 1005.87 μg mL -1, respectively, for the decrease in cupric/COF-mediated SC plasmid DNA damage. The boiled water extract has the best protective effect. The ethyl acetate and ether extracts did not inhibit plasmid DNA damage. By using the comet assay, IC50 values of the ether, methanol and acetone extracts from ACB were 672.95, 64.34, and 397.77 μg mL-1, respectively, for the decrease in COF-mediated DNA migration in CL-3 cells. The methanol extract had the best protective effect. However, water, boiled water and ethyl acetate extracts from ACB showed no protective effect on COF-mediated DNA migration. These results indicate that the protective capacity of ACES on DNA damage induced by environmental mutagens is different in pUC18 plasmid DNA and CL-3 cell DNA.
原文英語
頁(從 - 到)1308-1316
頁數9
期刊Journal of the Science of Food and Agriculture
86
發行號9
DOIs
出版狀態已發佈 - 7月 2006
對外發佈

ASJC Scopus subject areas

  • 農業與生物科學(雜項)
  • 食品科學
  • 化學(雜項)

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