摘要
Ethnopharmacological relevance: Osteoporosis is one of the most common bone diseases; it is characterized by bone loss and is a risk factor for hip fracture. Chinese herbal medicines (CHMs) and their related natural compounds have been used for treating many diseases, including bone diseases, since ancient times in China and are regarded as a cost-effective complementary therapy. Aim of the study: The goal of this study was to investigate the osteoprotective mechanisms of these three Chinese herbs and their related natural compounds. The effects of CHMs and related natural compounds on RANKL-induced osteoclastogenesis in vitro were investigated. Materials and methods: A network pharmacology method was applied to study CHM-related natural compounds and their osteoporosis targets. In addition, their effect on RANKL-induced osteoclastogenesis in RAW264.7 cells was also investigated in vitro. Results: Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae exhibited protective effects against mortality in hip fracture patients. Furthermore, these three herbs inhibited RANKL-induced TRAP activities and reduced the expression of bone resorption-related genes in RAW264.7 cells. Network analysis of natural compound (ingredient)-target interactions identified 11 natural compounds. Signal pathway analyses suggested that these compounds may target cytokine-cytokine receptor interactions, including RANKL-induced osteoclastogenesis. Five novel natural compounds exhibited reduced RANKL-induced TRAP activities and bone resorption-related gene expression. Conclusion: The clinically used CHMs, Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae, and natural compounds obtained from them may suppress RANKL-induced osteoclastogenesis in vitro.
原文 | 英語 |
---|---|
文章編號 | 112074 |
期刊 | Journal of Ethnopharmacology |
卷 | 244 |
DOIs | |
出版狀態 | 已發佈 - 十一月 15 2019 |
指紋
ASJC Scopus subject areas
- Pharmacology
- Drug Discovery
引用此文
Protective effects and network analysis of natural compounds obtained from Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae against RANKL-induced osteoclastogenesis in vitro. / Cheng, Chi Fung; Chien-Fu Lin, Jeff; Tsai, Fuu Jen; Chen, Chao Jung; Chiou, Jian Shiun; Chou, Chen Hsing; Li, Te Mao; Lin, Ting Hsu; Liao, Chiu Chu; Huang, Shao Mei; Li, Ju Pi; Lin, Jung Chun; Lin, Chih Chien; Ban, Bo; Liang, Wen Miin; Lin, Ying Ju.
於: Journal of Ethnopharmacology, 卷 244, 112074, 15.11.2019.研究成果: 雜誌貢獻 › 文章
}
TY - JOUR
T1 - Protective effects and network analysis of natural compounds obtained from Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae against RANKL-induced osteoclastogenesis in vitro
AU - Cheng, Chi Fung
AU - Chien-Fu Lin, Jeff
AU - Tsai, Fuu Jen
AU - Chen, Chao Jung
AU - Chiou, Jian Shiun
AU - Chou, Chen Hsing
AU - Li, Te Mao
AU - Lin, Ting Hsu
AU - Liao, Chiu Chu
AU - Huang, Shao Mei
AU - Li, Ju Pi
AU - Lin, Jung Chun
AU - Lin, Chih Chien
AU - Ban, Bo
AU - Liang, Wen Miin
AU - Lin, Ying Ju
PY - 2019/11/15
Y1 - 2019/11/15
N2 - Ethnopharmacological relevance: Osteoporosis is one of the most common bone diseases; it is characterized by bone loss and is a risk factor for hip fracture. Chinese herbal medicines (CHMs) and their related natural compounds have been used for treating many diseases, including bone diseases, since ancient times in China and are regarded as a cost-effective complementary therapy. Aim of the study: The goal of this study was to investigate the osteoprotective mechanisms of these three Chinese herbs and their related natural compounds. The effects of CHMs and related natural compounds on RANKL-induced osteoclastogenesis in vitro were investigated. Materials and methods: A network pharmacology method was applied to study CHM-related natural compounds and their osteoporosis targets. In addition, their effect on RANKL-induced osteoclastogenesis in RAW264.7 cells was also investigated in vitro. Results: Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae exhibited protective effects against mortality in hip fracture patients. Furthermore, these three herbs inhibited RANKL-induced TRAP activities and reduced the expression of bone resorption-related genes in RAW264.7 cells. Network analysis of natural compound (ingredient)-target interactions identified 11 natural compounds. Signal pathway analyses suggested that these compounds may target cytokine-cytokine receptor interactions, including RANKL-induced osteoclastogenesis. Five novel natural compounds exhibited reduced RANKL-induced TRAP activities and bone resorption-related gene expression. Conclusion: The clinically used CHMs, Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae, and natural compounds obtained from them may suppress RANKL-induced osteoclastogenesis in vitro.
AB - Ethnopharmacological relevance: Osteoporosis is one of the most common bone diseases; it is characterized by bone loss and is a risk factor for hip fracture. Chinese herbal medicines (CHMs) and their related natural compounds have been used for treating many diseases, including bone diseases, since ancient times in China and are regarded as a cost-effective complementary therapy. Aim of the study: The goal of this study was to investigate the osteoprotective mechanisms of these three Chinese herbs and their related natural compounds. The effects of CHMs and related natural compounds on RANKL-induced osteoclastogenesis in vitro were investigated. Materials and methods: A network pharmacology method was applied to study CHM-related natural compounds and their osteoporosis targets. In addition, their effect on RANKL-induced osteoclastogenesis in RAW264.7 cells was also investigated in vitro. Results: Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae exhibited protective effects against mortality in hip fracture patients. Furthermore, these three herbs inhibited RANKL-induced TRAP activities and reduced the expression of bone resorption-related genes in RAW264.7 cells. Network analysis of natural compound (ingredient)-target interactions identified 11 natural compounds. Signal pathway analyses suggested that these compounds may target cytokine-cytokine receptor interactions, including RANKL-induced osteoclastogenesis. Five novel natural compounds exhibited reduced RANKL-induced TRAP activities and bone resorption-related gene expression. Conclusion: The clinically used CHMs, Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae, and natural compounds obtained from them may suppress RANKL-induced osteoclastogenesis in vitro.
KW - Chinese herbal medicine
KW - Hip fracture
KW - Natural compound
KW - Osteoclastogenesis
KW - Osteoporosis
UR - http://www.scopus.com/inward/record.url?scp=85070103941&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85070103941&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2019.112074
DO - 10.1016/j.jep.2019.112074
M3 - Article
C2 - 31291608
AN - SCOPUS:85070103941
VL - 244
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
SN - 0378-8741
M1 - 112074
ER -