Protective effects and network analysis of natural compounds obtained from Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae against RANKL-induced osteoclastogenesis in vitro

Chi Fung Cheng, Jeff Chien-Fu Lin, Fuu Jen Tsai, Chao Jung Chen, Jian Shiun Chiou, Chen Hsing Chou, Te Mao Li, Ting Hsu Lin, Chiu Chu Liao, Shao Mei Huang, Ju Pi Li, Jung Chun Lin, Chih Chien Lin, Bo Ban, Wen Miin Liang, Ying Ju Lin

研究成果: 雜誌貢獻 › 文章

2 引文 (Scopus)

摘要

Ethnopharmacological relevance: Osteoporosis is one of the most common bone diseases; it is characterized by bone loss and is a risk factor for hip fracture. Chinese herbal medicines (CHMs) and their related natural compounds have been used for treating many diseases, including bone diseases, since ancient times in China and are regarded as a cost-effective complementary therapy. Aim of the study: The goal of this study was to investigate the osteoprotective mechanisms of these three Chinese herbs and their related natural compounds. The effects of CHMs and related natural compounds on RANKL-induced osteoclastogenesis in vitro were investigated. Materials and methods: A network pharmacology method was applied to study CHM-related natural compounds and their osteoporosis targets. In addition, their effect on RANKL-induced osteoclastogenesis in RAW264.7 cells was also investigated in vitro. Results: Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae exhibited protective effects against mortality in hip fracture patients. Furthermore, these three herbs inhibited RANKL-induced TRAP activities and reduced the expression of bone resorption-related genes in RAW264.7 cells. Network analysis of natural compound (ingredient)-target interactions identified 11 natural compounds. Signal pathway analyses suggested that these compounds may target cytokine-cytokine receptor interactions, including RANKL-induced osteoclastogenesis. Five novel natural compounds exhibited reduced RANKL-induced TRAP activities and bone resorption-related gene expression. Conclusion: The clinically used CHMs, Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae, and natural compounds obtained from them may suppress RANKL-induced osteoclastogenesis in vitro.

原文英語
文章編號112074
期刊Journal of Ethnopharmacology
卷244
DOIs
出版狀態已發佈 - 十一月 15 2019

指紋

Polypodiaceae
Herbal Medicine
Osteogenesis
Bone Diseases
Hip Fractures
Bone Resorption
Osteoporosis
Cytokine Receptors
Complementary Therapies
Signal Transduction
China
Pharmacology
Cytokines
Gene Expression
Costs and Cost Analysis
Bone and Bones
Mortality
In Vitro Techniques
Genes

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

引用此文

Protective effects and network analysis of natural compounds obtained from Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae against RANKL-induced osteoclastogenesis in vitro. / Cheng, Chi Fung; Chien-Fu Lin, Jeff; Tsai, Fuu Jen; Chen, Chao Jung; Chiou, Jian Shiun; Chou, Chen Hsing; Li, Te Mao; Lin, Ting Hsu; Liao, Chiu Chu; Huang, Shao Mei; Li, Ju Pi; Lin, Jung Chun; Lin, Chih Chien; Ban, Bo; Liang, Wen Miin; Lin, Ying Ju.

於: Journal of Ethnopharmacology, 卷 244, 112074, 15.11.2019.

研究成果: 雜誌貢獻 › 文章

Cheng, CF, Chien-Fu Lin, J, Tsai, FJ, Chen, CJ, Chiou, JS, Chou, CH, Li, TM, Lin, TH, Liao, CC, Huang, SM, Li, JP, Lin, JC, Lin, CC, Ban, B, Liang, WM & Lin, YJ 2019, 'Protective effects and network analysis of natural compounds obtained from Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae against RANKL-induced osteoclastogenesis in vitro', Journal of Ethnopharmacology, 卷 244, 112074. https://doi.org/10.1016/j.jep.2019.112074
Cheng CF, Chien-Fu Lin J, Tsai FJ, Chen CJ, Chiou JS, Chou CH 等. Protective effects and network analysis of natural compounds obtained from Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae against RANKL-induced osteoclastogenesis in vitro. Journal of Ethnopharmacology. 2019 11月 15;244. 112074. https://doi.org/10.1016/j.jep.2019.112074
Cheng, Chi Fung ; Chien-Fu Lin, Jeff ; Tsai, Fuu Jen ; Chen, Chao Jung ; Chiou, Jian Shiun ; Chou, Chen Hsing ; Li, Te Mao ; Lin, Ting Hsu ; Liao, Chiu Chu ; Huang, Shao Mei ; Li, Ju Pi ; Lin, Jung Chun ; Lin, Chih Chien ; Ban, Bo ; Liang, Wen Miin ; Lin, Ying Ju. / Protective effects and network analysis of natural compounds obtained from Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae against RANKL-induced osteoclastogenesis in vitro. 於: Journal of Ethnopharmacology. 2019 ; 卷 244.
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abstract = "Ethnopharmacological relevance: Osteoporosis is one of the most common bone diseases; it is characterized by bone loss and is a risk factor for hip fracture. Chinese herbal medicines (CHMs) and their related natural compounds have been used for treating many diseases, including bone diseases, since ancient times in China and are regarded as a cost-effective complementary therapy. Aim of the study: The goal of this study was to investigate the osteoprotective mechanisms of these three Chinese herbs and their related natural compounds. The effects of CHMs and related natural compounds on RANKL-induced osteoclastogenesis in vitro were investigated. Materials and methods: A network pharmacology method was applied to study CHM-related natural compounds and their osteoporosis targets. In addition, their effect on RANKL-induced osteoclastogenesis in RAW264.7 cells was also investigated in vitro. Results: Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae exhibited protective effects against mortality in hip fracture patients. Furthermore, these three herbs inhibited RANKL-induced TRAP activities and reduced the expression of bone resorption-related genes in RAW264.7 cells. Network analysis of natural compound (ingredient)-target interactions identified 11 natural compounds. Signal pathway analyses suggested that these compounds may target cytokine-cytokine receptor interactions, including RANKL-induced osteoclastogenesis. Five novel natural compounds exhibited reduced RANKL-induced TRAP activities and bone resorption-related gene expression. Conclusion: The clinically used CHMs, Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae, and natural compounds obtained from them may suppress RANKL-induced osteoclastogenesis in vitro.",
keywords = "Chinese herbal medicine, Hip fracture, Natural compound, Osteoclastogenesis, Osteoporosis",
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T1 - Protective effects and network analysis of natural compounds obtained from Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae against RANKL-induced osteoclastogenesis in vitro

AU - Cheng, Chi Fung

AU - Chien-Fu Lin, Jeff

AU - Tsai, Fuu Jen

AU - Chen, Chao Jung

AU - Chiou, Jian Shiun

AU - Chou, Chen Hsing

AU - Li, Te Mao

AU - Lin, Ting Hsu

AU - Liao, Chiu Chu

AU - Huang, Shao Mei

AU - Li, Ju Pi

AU - Lin, Jung Chun

AU - Lin, Chih Chien

AU - Ban, Bo

AU - Liang, Wen Miin

AU - Lin, Ying Ju

PY - 2019/11/15

Y1 - 2019/11/15

N2 - Ethnopharmacological relevance: Osteoporosis is one of the most common bone diseases; it is characterized by bone loss and is a risk factor for hip fracture. Chinese herbal medicines (CHMs) and their related natural compounds have been used for treating many diseases, including bone diseases, since ancient times in China and are regarded as a cost-effective complementary therapy. Aim of the study: The goal of this study was to investigate the osteoprotective mechanisms of these three Chinese herbs and their related natural compounds. The effects of CHMs and related natural compounds on RANKL-induced osteoclastogenesis in vitro were investigated. Materials and methods: A network pharmacology method was applied to study CHM-related natural compounds and their osteoporosis targets. In addition, their effect on RANKL-induced osteoclastogenesis in RAW264.7 cells was also investigated in vitro. Results: Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae exhibited protective effects against mortality in hip fracture patients. Furthermore, these three herbs inhibited RANKL-induced TRAP activities and reduced the expression of bone resorption-related genes in RAW264.7 cells. Network analysis of natural compound (ingredient)-target interactions identified 11 natural compounds. Signal pathway analyses suggested that these compounds may target cytokine-cytokine receptor interactions, including RANKL-induced osteoclastogenesis. Five novel natural compounds exhibited reduced RANKL-induced TRAP activities and bone resorption-related gene expression. Conclusion: The clinically used CHMs, Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae, and natural compounds obtained from them may suppress RANKL-induced osteoclastogenesis in vitro.

AB - Ethnopharmacological relevance: Osteoporosis is one of the most common bone diseases; it is characterized by bone loss and is a risk factor for hip fracture. Chinese herbal medicines (CHMs) and their related natural compounds have been used for treating many diseases, including bone diseases, since ancient times in China and are regarded as a cost-effective complementary therapy. Aim of the study: The goal of this study was to investigate the osteoprotective mechanisms of these three Chinese herbs and their related natural compounds. The effects of CHMs and related natural compounds on RANKL-induced osteoclastogenesis in vitro were investigated. Materials and methods: A network pharmacology method was applied to study CHM-related natural compounds and their osteoporosis targets. In addition, their effect on RANKL-induced osteoclastogenesis in RAW264.7 cells was also investigated in vitro. Results: Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae exhibited protective effects against mortality in hip fracture patients. Furthermore, these three herbs inhibited RANKL-induced TRAP activities and reduced the expression of bone resorption-related genes in RAW264.7 cells. Network analysis of natural compound (ingredient)-target interactions identified 11 natural compounds. Signal pathway analyses suggested that these compounds may target cytokine-cytokine receptor interactions, including RANKL-induced osteoclastogenesis. Five novel natural compounds exhibited reduced RANKL-induced TRAP activities and bone resorption-related gene expression. Conclusion: The clinically used CHMs, Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae, and natural compounds obtained from them may suppress RANKL-induced osteoclastogenesis in vitro.

KW - Chinese herbal medicine

KW - Hip fracture

KW - Natural compound

KW - Osteoclastogenesis

KW - Osteoporosis

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