Protective effect of low-molecular-weight fucoidan on radiation-induced fibrosis through tgf-β1/smad pathway-mediated inhibition of collagen i accumulation

Szu Yuan Wu, Yu Ting Chen, Guo Yu Tsai, Fu Yin Hsu, Pai An Hwang

研究成果: 雜誌貢獻文章

1 引文 斯高帕斯(Scopus)

摘要

Radiation-induced fibrosis (RIF) occurs after radiation therapy in normal tissues due to excessive production and deposition of extracellular matrix proteins and collagen, possibly resulting in organ function impairment. This study investigates the effects of low-molecular-weight fucoidan (LMF) on irradiated NIH3T3 cells. Specifically, we quantified cellular metabolic activity, fibrosis-related mRNA expression, transforming growth factor beta-1 (TGF-β1), and collagen-1 protein expression, and fibroblast contractility in response to LMF. LMF pre + post-treatment could more effectively increase cellular metabolic activity compared with LMF post-treatment. LMF pre + post-treatment inhibited TGF-β1 expression, which mediates negative activation of phosphorylated Smad3 (pSmad3) and Smad4 complex formation and suppresses downstream collagen I accumulation. In addition, LMF pre + post-treatment significantly reduced actin-stress fibers in irradiated NIH3T3 cells. LMF, a natural substance obtained from brown seaweed, may be a candidate agent for preventing or inhibiting RIF.

原文英語
文章編號md18030136
期刊Marine Drugs
18
發行號3
DOIs
出版狀態已發佈 - 一月 1 2020
對外發佈Yes

ASJC Scopus subject areas

  • Drug Discovery

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