Propofol suppresses tumor necrosis factor-α biosynthesis in lipopolysaccharide-stimulated macrophages possibly through downregulation of nuclear factor-kappa B-mediated toll-like receptor 4 gene expression

研究成果: 雜誌貢獻文章

32 引文 斯高帕斯(Scopus)

摘要

Lipopolysaccharide (LPS), a gram-negative bacterial outer membrane component, can activate macrophages via a toll-like receptor 4-dependent pathway. Our previous study has shown that propofol, an intravenous anesthetic reagent, has anti-inflammatory effects. This study was further aimed to evaluate the roles of toll-like receptor 4 in propofol-caused suppression of tumor necrosis factor-α (TNF-α) biosynthesis in LPS-stimulated macrophages and its possible molecular mechanisms. Exposure of macrophages to propofol and LPS did not affect cell viability. Meanwhile, the LPS-caused augmentations in the productions of TNF-α protein and mRNA were significantly decreased following incubation with a therapeutic concentration of propofol (50 μM). Analysis of toll-like receptor 4 small interference (si)RNA revealed that this membrane receptor might participate in the propofol-caused suppression of TNF-α biosynthesis. Treatment of macrophages with LPS-induced toll-like receptor 4 protein and mRNA productions. Propofol at a clinically relevant concentration could inhibit such induction. In parallel, the LPS-induced translocation and transactivation of transcription factor nuclear factor-kappa B (NFκB) were significantly alleviated following propofol incubation. There are several NFκB DNA-binding motifs found in the promoter region of toll-like receptor 4. Therefore, this study shows that propofol at a therapeutic concentration can downregulate TNF-α biosynthesis possibly via inhibition of NFκB-mediated toll-like receptor 4 gene expression.

原文英語
頁(從 - 到)465-471
頁數7
期刊Chemico-Biological Interactions
180
發行號3
DOIs
出版狀態已發佈 - 八月 14 2009

ASJC Scopus subject areas

  • Toxicology

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