Promoter methylation of SFRP3 is frequent in hepatocellular carcinoma

Ya Wen Lin, Yu Lueng Shih, Gi Shih Lien, Fat Moon Suk, Chung Bao Hsieh, Ming De Yan

研究成果: 雜誌貢獻文章同行評審

13 引文 斯高帕斯(Scopus)

摘要

Oncogenic activation of the Wnt/β-catenin signaling pathway is common in human cancers. The secreted frizzled-related proteins (SFRPs) function as negative regulators of Wnt signaling and have important implications in carcinogenesis. Because there have been no reports about the role of SFRP3 in hepatocellular carcinoma (HCC), we investigated the level of methylation and transcription of SFRP3. Four HCC cell lines, 60 HCCs, 23 cirrhosis livers, 37 chronic hepatitis livers, and 30 control livers were prescreened for SFRP3 promoter methylation by methylation-specific polymerase chain reaction (MS-PCR) and bisulfite sequencing. SFRP3 promoter methylation was observed in 100%, 60%, 39.1%, 16.2%, and 0% in HCC cell lines, primary HCCs, cirrhosis livers, chronic hepatitis livers, and control livers, respectively. Demethylation treatment with 5-aza-2′-deoxycytidine in HCC cells restored or increased the SFRP3 mRNA expression. We next used quantitative MS-PCR (QMSP) to analyze the methylation level of SFRP3 in 60 HCCs and their corresponding nontumor tissues. Methylation of SFRP3 promoter region in HCCs increased significantly compared with control tissues. There is a positive correlation between promoter hypermethylation and SFRP3 mRNA downregulation. Our data suggest that promoter hypermethylation of SFRP3 is a common event in HCCs and plays an important role in regulation of SFRP3 mRNA expression.
原文英語
文章編號351863
期刊Disease Markers
2014
DOIs
出版狀態已發佈 - 2014

ASJC Scopus subject areas

  • 生物化學(醫學)
  • 臨床生物化學
  • 分子生物學
  • 遺傳學
  • 醫藥 (全部)

指紋

深入研究「Promoter methylation of SFRP3 is frequent in hepatocellular carcinoma」主題。共同形成了獨特的指紋。

引用此