Promising urinary miRNA biomarkers t-SPR profiling for urothelial cell carcinoma

Wing Kiu Yeung, Hsiao Fen Li, Cheng Lin Chung, Kuang Li Lee, Pei Kuen Wei, Heng Lin, Hsi Hsien Chen, Ji Yen Cheng

研究成果: 雜誌貢獻文章同行評審

2 引文 斯高帕斯(Scopus)

摘要

An integrated transmission surface plasmon resonance platform with microfluidic sensor has a high potential to be a medical diagnostic tool. There has been extensive research into the potential use of transmission surface plasmon resonance, but here we demonstrate the practical application of transmission surface plasmon resonance to monitor and compare the urinary microRNA expression between the urothelial cell carcinoma patient group and the healthy control group. Furthermore, the expression levels are compared with the conventional real-time quantitative polymerase chain reaction method to verify the results. The relative expression of miR-21–3p and miR–214–3p have demonstrated to be upregulated in urothelial cell carcinoma patient group when measured using both real-time quantitative polymerase chain reaction and transmission surface plasmon resonance. Using receiver operating characteristics of miR-21–3p and miR-214–3p the area under curve reaches an excellent score of 0.99 and 0.917, respectively. Our work has demonstrated the ability of clinical application of transmission surface plasmon resonance as a rapid and cost effective point-of-care.
原文英語
文章編號128605
期刊Sensors and Actuators, B: Chemical
322
DOIs
出版狀態已發佈 - 十一月 1 2020

ASJC Scopus subject areas

  • 電子、光磁材料
  • 儀器
  • 凝聚態物理學
  • 表面、塗料和薄膜
  • 金屬和合金
  • 電氣與電子工程
  • 材料化學

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