Process development of an acellular dermal matrix (ADM) for biomedical applications

Ray Neng Chen, Hsiu O. Ho, Yu Ting Tsai, Ming Thau Sheu

研究成果: 雜誌貢獻文章同行評審

210 引文 斯高帕斯(Scopus)

摘要

The object of this study was to compare the extent of decellularization at each critical step of processing porcine skin to produce an acellular dermal matrix (ADM) for biomedical applications. The results demonstrated that the removal of epidermis using treatment with 0.25% trypsin for 18h and 0.1% sodium dodecyl sulfate (SDS) for 12h at room temperature was beneficial for the subsequent treatment to remove cells in the dermal structure. Lengthy incubation in 0.25% trypsin (12h) and then 560units/l Dispase (12h) at 25°C of small pieces of porcine skin from which the epidermis had been removed efficiently removed cells and cellular components from the skin. Histological examinations revealed that the epidermis, dermal fibroblasts, and epidermal appendages were completely removed by these treatments, and the basic dermal architecture of collagen bundles was that of a loose meshwork. Examinations by TEM showed that the characteristics of collagen fibers in the ADM were retained after complete removal of cells present under optimal conditions defined in this study. SDS-PAGE and size-exclusion HPLC revealed that collagen fibers in the ADM were mostly type I and showed two typical component peaks identified as oligomers and monomers, respectively.

原文英語
頁(從 - 到)2679-2686
頁數8
期刊Biomaterials
25
發行號13
DOIs
出版狀態已發佈 - 6月 2004

ASJC Scopus subject areas

  • 生物工程
  • 陶瓷和複合材料
  • 生物物理學
  • 生物材料
  • 材料力學

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