Primers specific for the fimbrial major subunit gene stdA can be used to detect Salmonella enterica serovars

Yin Ching Chuang, Chia Huei Yang, Jiunn Horng Lin, Ke Chuan Wang, Chun Ping Cheng, Kuang Sheng Yeh

研究成果: 雜誌貢獻文章同行評審

2 引文 斯高帕斯(Scopus)

摘要

The feasibility of using two primers internal to the stdA gene (which encodes the fimbrial major subunit of the std fimbrial gene cluster in Salmonella enterica serovar Typhi) to detect Salmonella by PCR was explored. The 518-bp stdA specific sequence was conserved among 268 strains from 45 serovars of S. enterica. One Salmonella bongori CCUG 30042 strain and 34 non-Salmonella strains did not possess this sequence. A sensitivity test revealed that the stdA-specific primer set detected 3.4 × 10_1 pg of genomic DNA and 3.0 × 105 CFU/ml with serial dilutions of Salmonella Typhimurium cells. In vitro testing for specificity using pig carcass sponge samples contaminated with Salmonella Typhimurium also was performed. An initial Salmonella Typhimurium inoculum of 4.4 × 101 CFU/ml in pig carcass exudates reached the stdA primer detection level after preenrichment in buffered peptone water at 37°C for 18 h in the presence of indigenous non-Salmonella flora at 4.0 × 107 CFU/ml, but the detection level decreased to 4.4 × 10° CFU/ml after selective enrichment in Rappaport-Vassiliadis R10 broth for 18 h at 42°C. The PCR method with primers specific for stdA is a quick and sensitive tool for detecting S. enterica, which is an important cause of foodborne disease.
原文英語
頁(從 - 到)1108-1113
頁數6
期刊Journal of Food Protection
71
發行號6
出版狀態已發佈 - 六月 2008

ASJC Scopus subject areas

  • 食品科學
  • 應用微生物與生物技術
  • 生物技術

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