Preservation of clinical isolates of Mycobacterium tuberculosis complex directly from MGIT culture tubes

Tsi S. Huang, Yao Shen Chen, S. S J Lee, Hui Z. Tu, Yung Ching Liu

研究成果: 雜誌貢獻文章同行評審

6 引文 斯高帕斯(Scopus)

摘要

Preservation of M. tuberculosis complex strains isolated from clinical specimens is important for epidemiological investigations related to tuberculosis. In this study the efficacy of preservation was evaluated by calculating the recovery rate of preserved strains, with various patterns of resistance, after periods of storage and subculture. The recovery rates from strains preserved in enriched solid medium were >90% for storage periods ≤6 yr. However, this procedure for storing mycobacteria is time-consuming, labor-intensive, and impractical for routine use in a clinical laboratory setting. This study shows that recovery rates for strains preserved directly from MGIT fluids are satisfactory for storage periods ≤2 yr. No significant difference in viability was observed within 3 categories of drug resistance: (i) all-susceptible, (ii) multi-drug resistant (MDR), and (iii) a combination of other patterns of resistance. Preserving clinical M. tuberculosis strains directly from MGIT culture fluid fits easily into laboratory routine and is feasible for use in a clinical laboratory setting.

原文英語
頁(從 - 到)455-458
頁數4
期刊Annals of Clinical and Laboratory Science
35
發行號4
出版狀態已發佈 - 9月 2005
對外發佈

ASJC Scopus subject areas

  • 生物化學、遺傳與分子生物學 (全部)
  • 臨床生物化學

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