Preparation of radioiodinated secretin for radioimmtmoassay

Chung-Huei Hsu, Kuo Wei Yin, Tsai Hsia Hong, Tswen Kei Tang, Long Shyong Lee, Tong H. Chang, Jean-Dean Liu, P. H. Chen

研究成果: 雜誌貢獻文章

摘要

Radioiodination of synthetic human secretin on its N-terminal histidyl residue was not difficult when a greater amount of Chloramine T and a longer reaction time were employed to achieve better incorporation of125I. The radioiodinated tracer for an optimal radioimmunoassay required purification. The combination of Sep-pak C18 Cartridge and high performance liquid chromatography for the purification of125I-secretin in our study revealed that the Sep-pak cartridge was a preliminary step in removing unlabeled radioactive iodide, the reactant, and labeled materials unadsorbed to the cartridge. The eluate eluted from the Sep-pak containing high radioactivity and high immunoreactivity to the antibody were selected for further purification by HPLC which eliminated undesirable radiolabeled substances with lower immunoreactivity. The purified radiolabeled secretin was used in developing a sensitive radioimmunoassay.
原文英語
頁(從 - 到)83-87
頁數5
期刊Annals of Nuclear Medicine
5
發行號3
DOIs
出版狀態已發佈 - 九月 1991
對外發佈Yes

指紋

Secretin
Radioimmunoassay
High Pressure Liquid Chromatography
Iodides
Radioactivity
Antibodies

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

引用此文

Hsu, C-H., Yin, K. W., Hong, T. H., Tang, T. K., Lee, L. S., Chang, T. H., ... Chen, P. H. (1991). Preparation of radioiodinated secretin for radioimmtmoassay. Annals of Nuclear Medicine, 5(3), 83-87. https://doi.org/10.1007/BF03164619

Preparation of radioiodinated secretin for radioimmtmoassay. / Hsu, Chung-Huei; Yin, Kuo Wei; Hong, Tsai Hsia; Tang, Tswen Kei; Lee, Long Shyong; Chang, Tong H.; Liu, Jean-Dean; Chen, P. H.

於: Annals of Nuclear Medicine, 卷 5, 編號 3, 09.1991, p. 83-87.

研究成果: 雜誌貢獻文章

Hsu, C-H, Yin, KW, Hong, TH, Tang, TK, Lee, LS, Chang, TH, Liu, J-D & Chen, PH 1991, 'Preparation of radioiodinated secretin for radioimmtmoassay', Annals of Nuclear Medicine, 卷 5, 編號 3, 頁 83-87. https://doi.org/10.1007/BF03164619
Hsu, Chung-Huei ; Yin, Kuo Wei ; Hong, Tsai Hsia ; Tang, Tswen Kei ; Lee, Long Shyong ; Chang, Tong H. ; Liu, Jean-Dean ; Chen, P. H. / Preparation of radioiodinated secretin for radioimmtmoassay. 於: Annals of Nuclear Medicine. 1991 ; 卷 5, 編號 3. 頁 83-87.
@article{ee31fa68b73d49478957d5bf265c915d,
title = "Preparation of radioiodinated secretin for radioimmtmoassay",
abstract = "Radioiodination of synthetic human secretin on its N-terminal histidyl residue was not difficult when a greater amount of Chloramine T and a longer reaction time were employed to achieve better incorporation of125I. The radioiodinated tracer for an optimal radioimmunoassay required purification. The combination of Sep-pak C18 Cartridge and high performance liquid chromatography for the purification of125I-secretin in our study revealed that the Sep-pak cartridge was a preliminary step in removing unlabeled radioactive iodide, the reactant, and labeled materials unadsorbed to the cartridge. The eluate eluted from the Sep-pak containing high radioactivity and high immunoreactivity to the antibody were selected for further purification by HPLC which eliminated undesirable radiolabeled substances with lower immunoreactivity. The purified radiolabeled secretin was used in developing a sensitive radioimmunoassay.",
keywords = "HPLC, radioimmunoassay, radioiodination, secretin",
author = "Chung-Huei Hsu and Yin, {Kuo Wei} and Hong, {Tsai Hsia} and Tang, {Tswen Kei} and Lee, {Long Shyong} and Chang, {Tong H.} and Jean-Dean Liu and Chen, {P. H.}",
year = "1991",
month = "9",
doi = "10.1007/BF03164619",
language = "English",
volume = "5",
pages = "83--87",
journal = "Annals of Nuclear Medicine",
issn = "0914-7187",
publisher = "Springer Japan",
number = "3",

}

TY - JOUR

T1 - Preparation of radioiodinated secretin for radioimmtmoassay

AU - Hsu, Chung-Huei

AU - Yin, Kuo Wei

AU - Hong, Tsai Hsia

AU - Tang, Tswen Kei

AU - Lee, Long Shyong

AU - Chang, Tong H.

AU - Liu, Jean-Dean

AU - Chen, P. H.

PY - 1991/9

Y1 - 1991/9

N2 - Radioiodination of synthetic human secretin on its N-terminal histidyl residue was not difficult when a greater amount of Chloramine T and a longer reaction time were employed to achieve better incorporation of125I. The radioiodinated tracer for an optimal radioimmunoassay required purification. The combination of Sep-pak C18 Cartridge and high performance liquid chromatography for the purification of125I-secretin in our study revealed that the Sep-pak cartridge was a preliminary step in removing unlabeled radioactive iodide, the reactant, and labeled materials unadsorbed to the cartridge. The eluate eluted from the Sep-pak containing high radioactivity and high immunoreactivity to the antibody were selected for further purification by HPLC which eliminated undesirable radiolabeled substances with lower immunoreactivity. The purified radiolabeled secretin was used in developing a sensitive radioimmunoassay.

AB - Radioiodination of synthetic human secretin on its N-terminal histidyl residue was not difficult when a greater amount of Chloramine T and a longer reaction time were employed to achieve better incorporation of125I. The radioiodinated tracer for an optimal radioimmunoassay required purification. The combination of Sep-pak C18 Cartridge and high performance liquid chromatography for the purification of125I-secretin in our study revealed that the Sep-pak cartridge was a preliminary step in removing unlabeled radioactive iodide, the reactant, and labeled materials unadsorbed to the cartridge. The eluate eluted from the Sep-pak containing high radioactivity and high immunoreactivity to the antibody were selected for further purification by HPLC which eliminated undesirable radiolabeled substances with lower immunoreactivity. The purified radiolabeled secretin was used in developing a sensitive radioimmunoassay.

KW - HPLC

KW - radioimmunoassay

KW - radioiodination

KW - secretin

UR - http://www.scopus.com/inward/record.url?scp=0026072595&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026072595&partnerID=8YFLogxK

U2 - 10.1007/BF03164619

DO - 10.1007/BF03164619

M3 - Article

C2 - 1764343

AN - SCOPUS:0026072595

VL - 5

SP - 83

EP - 87

JO - Annals of Nuclear Medicine

JF - Annals of Nuclear Medicine

SN - 0914-7187

IS - 3

ER -