Preparation of 4-Deoxy- L -erythro-5-hexoseulose Uronic Acid (DEH) and Guluronic Acid Rich Alginate Using a Unique exo-Alginate Lyase from Thalassotalea crassostreae

Damao Wang, Olav A. Aarstad, Jing Li, Lauren S. McKee, Gerd Inger Sætrom, Anisha Vyas, Vaibhav Srivastava, Finn L. Aachmann, Vincent Bulone, Yves Sy Hsieh

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20 引文 斯高帕斯(Scopus)

摘要

Marine multicellular algae are considered promising crops for the production of sustainable biofuels and commodity chemicals. However, their commercial exploitation is currently limited by a lack of appropriate and efficient enzymes for converting alginate into metabolizable building blocks, such as 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH). Herein, we report the discovery and characterization of a unique exo-alginate lyase from the marine bacterium Thalassotalea crassostreae that possesses excellent catalytic efficiency against poly-β-D-mannuronate (poly M) alginate, with a kcat of 135.8 s-1, and a 5-fold lower kcat of 25 s-1 against poly-α-L-guluronate (poly G alginate). We propose that this preference for poly M is due to a structural feature of the protein's active site. The mode of action and specificity of this enzyme has made it possible to design an effective and environmentally friendly process for the production of DEH and low molecular weight guluronate-enriched alginate.

原文英語
頁(從 - 到)1435-1443
頁數9
期刊Journal of Agricultural and Food Chemistry
66
發行號6
DOIs
出版狀態已發佈 - 2月 14 2018
對外發佈

ASJC Scopus subject areas

  • 化學 (全部)
  • 農業與生物科學 (全部)

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