Preparation and characterization of nanoparticles shelled with chitosan for oral insulin delivery

Yu Hsin Lin, Fwu Long Mi, Chiung Tong Chen, Wei Chun Chang, Shu Fen Peng, Hsiang Fa Liang, Hsing Wen Sung

研究成果: 雜誌貢獻文章同行評審

274 引文 斯高帕斯(Scopus)

摘要

Nanoparticles (NPs) composed of chitosan (CS) and poly(γ-glutamic acid) (γ-PGA) were prepared by a simple ionic-gelation method for oral insulin delivery. Fourier transform infrared (FT-IR) spectra indicated that CS and γ-PGA were ionized at pH 2.5-6.6, while X-ray diffractograms demonstrated that the crystal structure of CS was disrupted after it was combined with γ-PGA. The diameters of the prepared NPs were in the range of 110-150 nm with a negative or positive surface charge, depending on the relative concentrations of CS to γ-PGA used. The NPs with a positive surface charge (or shelled with CS) could transiently open the tight junctions between Caco-2 cells and thus increased the paracellular permeability. After loading of insulin, the NPs remained spherical and the insulin release profiles were significantly affected by their stability in distinct pH environments. The in vivo results clearly indicated that the insulin-loaded NPs could effectively reduce the blood glucose level in a diabetic rat model.

原文英語
頁(從 - 到)146-152
頁數7
期刊Biomacromolecules
8
發行號1
DOIs
出版狀態已發佈 - 一月 2007
對外發佈Yes

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Polymers and Plastics
  • Materials Chemistry

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