Preparation and characterization of chemically TEMPO-oxidized and mechanically disintegrated sacchachitin nanofibers (SCNF) for enhanced diabetic wound healing

Fang Ching Chao, Meng Huang Wu, Ling Chun Chen, Hong Liang Lin, Der Zen Liu, Hsiu O. Ho, Ming Thau Sheu

研究成果: 雜誌貢獻文章

摘要

TEMPO-oxidization and mechanical disintegration were utilized to develop sacchachitin nanofibers (SCNF) with a 3D gel structure for being an ideal scaffold. Mechanically disintegrated SCNF (MDSCNF) with NanoLyzer® at 20,000 psi for 5 cycles and TEMPO-oxidized SCNF (TOSCNF) produced with 5.0 and 10.0 mmole NaClO/g SC was designated as SCN5, T050SC, and T100SC, respectively. All 2% MDSCNF suspensions were demonstrated to be in gel form, while all except T100SC of 2% TOSCNF suspensions showed to be wet fiber-like hydrogel. In diabetic wound healing study, both SCN5 and T050SC incorporated in AMPS (2-acrylamide-2-methyl-propane sulfonate)-based wound dressing were showed to accelerate diabetic wound healing forming nearly the same as normal tissues. T050SC/H further provided the healed wound with growth of sweat glands and hair follicles indicating the wound had healed as functional tissue. Conclusively, TEMPO-oxidized SCNF-based hydrogel scaffolds showed greater potentials in tissue regeneration due to its unique physical and chemical properties.

原文英語
文章編號115507
頁(從 - 到)115507
期刊Carbohydrate Polymers
229
DOIs
出版狀態已發佈 - 二月 1 2020

指紋

Nanofibers
Hydrogel
Hydrogels
Scaffolds
Suspensions
Gels
Tissue
Tissue regeneration
Propane
Acrylamide
Disintegration
Chemical properties
Physical properties
TEMPO
Fibers

ASJC Scopus subject areas

  • Materials Chemistry
  • Polymers and Plastics
  • Organic Chemistry

引用此文

@article{3d3d515d6c1d4214ac13a555f0805000,
title = "Preparation and characterization of chemically TEMPO-oxidized and mechanically disintegrated sacchachitin nanofibers (SCNF) for enhanced diabetic wound healing",
abstract = "TEMPO-oxidization and mechanical disintegration were utilized to develop sacchachitin nanofibers (SCNF) with a 3D gel structure for being an ideal scaffold. Mechanically disintegrated SCNF (MDSCNF) with NanoLyzer{\circledR} at 20,000 psi for 5 cycles and TEMPO-oxidized SCNF (TOSCNF) produced with 5.0 and 10.0 mmole NaClO/g SC was designated as SCN5, T050SC, and T100SC, respectively. All 2{\%} MDSCNF suspensions were demonstrated to be in gel form, while all except T100SC of 2{\%} TOSCNF suspensions showed to be wet fiber-like hydrogel. In diabetic wound healing study, both SCN5 and T050SC incorporated in AMPS (2-acrylamide-2-methyl-propane sulfonate)-based wound dressing were showed to accelerate diabetic wound healing forming nearly the same as normal tissues. T050SC/H further provided the healed wound with growth of sweat glands and hair follicles indicating the wound had healed as functional tissue. Conclusively, TEMPO-oxidized SCNF-based hydrogel scaffolds showed greater potentials in tissue regeneration due to its unique physical and chemical properties.",
keywords = "2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO), 2-acrylamido-2-methyl-1-propanesulfonic acid sodium salt (AMPS), Chitosan, Diabetic wound healing, Irgacure{\circledR} 184, L-ascorbic acid, poly(ethylene glycol) diacrylate, Mechanical disintegration, N,N-dimethylacrylamide, Nanofibers, PEG1000, PVP K90, Sacchachitin, Sodium bromide, Sodium hypochlorite, TEMPO-oxidation, Thiazolyl blue tetrazolium bromide (MTT reagent), chitosan, sodium bromide, thiazolyl blue tetrazolium bromide (MTT reagent)",
author = "Chao, {Fang Ching} and Wu, {Meng Huang} and Chen, {Ling Chun} and Lin, {Hong Liang} and Liu, {Der Zen} and Ho, {Hsiu O.} and Sheu, {Ming Thau}",
note = "Copyright {\circledC} 2019 Elsevier Ltd. All rights reserved.",
year = "2020",
month = "2",
day = "1",
doi = "10.1016/j.carbpol.2019.115507",
language = "English",
volume = "229",
pages = "115507",
journal = "Carbohydrate Polymers",
issn = "0144-8617",
publisher = "Elsevier Limited",

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TY - JOUR

T1 - Preparation and characterization of chemically TEMPO-oxidized and mechanically disintegrated sacchachitin nanofibers (SCNF) for enhanced diabetic wound healing

AU - Chao, Fang Ching

AU - Wu, Meng Huang

AU - Chen, Ling Chun

AU - Lin, Hong Liang

AU - Liu, Der Zen

AU - Ho, Hsiu O.

AU - Sheu, Ming Thau

N1 - Copyright © 2019 Elsevier Ltd. All rights reserved.

PY - 2020/2/1

Y1 - 2020/2/1

N2 - TEMPO-oxidization and mechanical disintegration were utilized to develop sacchachitin nanofibers (SCNF) with a 3D gel structure for being an ideal scaffold. Mechanically disintegrated SCNF (MDSCNF) with NanoLyzer® at 20,000 psi for 5 cycles and TEMPO-oxidized SCNF (TOSCNF) produced with 5.0 and 10.0 mmole NaClO/g SC was designated as SCN5, T050SC, and T100SC, respectively. All 2% MDSCNF suspensions were demonstrated to be in gel form, while all except T100SC of 2% TOSCNF suspensions showed to be wet fiber-like hydrogel. In diabetic wound healing study, both SCN5 and T050SC incorporated in AMPS (2-acrylamide-2-methyl-propane sulfonate)-based wound dressing were showed to accelerate diabetic wound healing forming nearly the same as normal tissues. T050SC/H further provided the healed wound with growth of sweat glands and hair follicles indicating the wound had healed as functional tissue. Conclusively, TEMPO-oxidized SCNF-based hydrogel scaffolds showed greater potentials in tissue regeneration due to its unique physical and chemical properties.

AB - TEMPO-oxidization and mechanical disintegration were utilized to develop sacchachitin nanofibers (SCNF) with a 3D gel structure for being an ideal scaffold. Mechanically disintegrated SCNF (MDSCNF) with NanoLyzer® at 20,000 psi for 5 cycles and TEMPO-oxidized SCNF (TOSCNF) produced with 5.0 and 10.0 mmole NaClO/g SC was designated as SCN5, T050SC, and T100SC, respectively. All 2% MDSCNF suspensions were demonstrated to be in gel form, while all except T100SC of 2% TOSCNF suspensions showed to be wet fiber-like hydrogel. In diabetic wound healing study, both SCN5 and T050SC incorporated in AMPS (2-acrylamide-2-methyl-propane sulfonate)-based wound dressing were showed to accelerate diabetic wound healing forming nearly the same as normal tissues. T050SC/H further provided the healed wound with growth of sweat glands and hair follicles indicating the wound had healed as functional tissue. Conclusively, TEMPO-oxidized SCNF-based hydrogel scaffolds showed greater potentials in tissue regeneration due to its unique physical and chemical properties.

KW - 2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO)

KW - 2-acrylamido-2-methyl-1-propanesulfonic acid sodium salt (AMPS)

KW - Chitosan

KW - Diabetic wound healing

KW - Irgacure® 184

KW - L-ascorbic acid, poly(ethylene glycol) diacrylate

KW - Mechanical disintegration

KW - N,N-dimethylacrylamide

KW - Nanofibers

KW - PEG1000

KW - PVP K90

KW - Sacchachitin

KW - Sodium bromide

KW - Sodium hypochlorite

KW - TEMPO-oxidation

KW - Thiazolyl blue tetrazolium bromide (MTT reagent)

KW - chitosan

KW - sodium bromide

KW - thiazolyl blue tetrazolium bromide (MTT reagent)

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UR - http://www.scopus.com/inward/citedby.url?scp=85075504602&partnerID=8YFLogxK

U2 - 10.1016/j.carbpol.2019.115507

DO - 10.1016/j.carbpol.2019.115507

M3 - Article

C2 - 31826505

AN - SCOPUS:85075504602

VL - 229

SP - 115507

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

M1 - 115507

ER -