Prenatal bisphenol a exposure, dna methylation, and low birth weight: A pilot study in taiwan

Yu Fang Huang, Chia Huang Chang, Pei Jung Chen, I. Hsuan Lin, Yen An Tsai, Chian Feng Chen, Yu Chao Wang, Wei Yun Huang, Ming Song Tsai, Mei Lien Chen

研究成果: 雜誌貢獻文章同行評審

摘要

Prenatal exposure to bisphenol A (BPA) may increase the risk of abnormal birth outcomes, and DNA methylation might mediate these adverse effects. This study aimed to investigate the effects of maternal BPA exposure on maternal and fetal DNA methylation levels and explore whether epigenetic changes are related to the associations between BPA and low birth weight. We collected urine and blood samples originating from 162 mother-infant pairs in a Taiwanese cohort study. We measured DNA methylation using the Illumina Infinium HumanMethylation 450 BeadChip in 34 maternal blood samples with high and low BPA levels based on the 75th percentile level (9.5 µg/g creatinine). Eighty-seven CpGs with the most differentially methylated probes possibly interacting with BPA exposure or birth weight were selected using two multiple regression models. Ingenuity pathway analysis (IPA) was utilized to narrow down 18 candidate CpGs related to disease categories, including developmental disorders, skeletal and muscular disorders, skeletal and muscular system development, metabolic diseases, and lipid metabolism. We then validated these genes by pyrose-quencing, and 8 CpGs met the primer design score requirements in 82 cord blood samples. The associations among low birth weight, BPA exposure, and DNA methylation were analyzed. Exposure to BPA was associated with low birth weight. Analysis of the epigenome-wide findings did not show significant associations between BPA and DNA methylation in cord blood of the 8 CpGs. However, the adjusted odds ratio for the dehydrogenase/reductase member 9 (DHRS9) gene, at the 2nd CG site, in the hypermethylated group was significantly associated with low birth weight. These results support a role of BPA, and possibly DHRS9 methylation, in fetal growth. However, additional studies with larger sample sizes are warranted.

原文英語
文章編號6144
期刊International journal of environmental research and public health
18
發行號11
DOIs
出版狀態已發佈 - 六月 1 2021

ASJC Scopus subject areas

  • 污染
  • 公共衛生、環境和職業健康
  • 健康、毒理學和誘變

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