Preconcentration enhanced immunoassay for protein sensing

Yu Jui Fan, Chih Zong Deng, Horn Jiunn Sheen

研究成果: 書貢獻/報告類型會議貢獻

摘要

This study reports on nanobeads immunosensing technique integrated with a nanofluidic preconcentration device to develop a rapid protein biosensor with high sensitivity and high selectivity. 300 nanobeads modified with antibodies were mixed with antigen and then pumped into a nanofluidic preconcentration device. After isolating the concentrated antigens and antibody-coated nanobeads, the micro-particle tracking velocimetry was used to measure the Brownian diffusion of the nanobeads in real-time to determine the antigen concentration. From this preconcentration experiment, we demonstrated that the rapid and high-sensitivity detection of biomarker can have a detection limit of 50 pg/ml in 20 min.
原文英語
主出版物標題21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
發行者Chemical and Biological Microsystems Society
頁面435-436
頁數2
ISBN(電子)9780692941836
出版狀態已發佈 - 一月 1 2020
事件21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, 美国
持續時間: 十月 22 2017十月 26 2017

出版系列

名字21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

會議

會議21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
國家美国
城市Savannah
期間10/22/1710/26/17

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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