This study reports on nanobeads immunosensing technique integrated with a nanofluidic preconcentration device to develop a rapid protein biosensor with high sensitivity and high selectivity. 300 nanobeads modified with antibodies were mixed with antigen and then pumped into a nanofluidic preconcentration device. After isolating the concentrated antigens and antibody-coated nanobeads, the micro-particle tracking velocimetry was used to measure the Brownian diffusion of the nanobeads in real-time to determine the antigen concentration. From this preconcentration experiment, we demonstrated that the rapid and high-sensitivity detection of biomarker can have a detection limit of 50 pg/ml in 20 min.
|名字||21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017|
|會議||21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017|
|期間||10/22/17 → 10/26/17|