Pravastatin induces thrombomodulin expression in TNFα-treated human aortic endothelial cells by inhibiting Rac1 and Cdc42 translocation and activity

Shing Jong Lin, Yung Hsiang Chen, Feng Yen Lin, Li Yuan Hsieh, Shu Huei Wang, Chia Ying Lin, Yu Chieh Wang, Hung Hai Ku, Jaw Wen Chen, Yuh Lien Chen

研究成果: 雜誌貢獻文章

18 引文 斯高帕斯(Scopus)

摘要

Expression of functionally active thrombomodulin (TM) on the luminal surface of endothelial cells is critical for vascular thromboresistance. The 3-hydroxyl-3-methyl coenzyme A reductase inhibitor, pravastatin, can protect the vasculature in a manner that is independent of its lipid-lowering activity. We examined the effect of pravastatin on TM expression by human aortic endothelial cells (HAECs) with subsequent tumor necrosis factor α (TNFα) stimulation and investigated the signaling pathways involved. TNFα treatment attenuated TM expression in HAECs in a time-dependent manner. Pravastatin upregulated TM levels in TNFα-treated HAECs. Specific inhibition of geranylgeranyl-transferase-I or the Rho family by GGTI-286 or TcdB, respectively, enhanced TM expression in TNFα-treated HAECs, whereas MAP kinase inhibitors, inactivation of Rho by Clostridium botulinum C3 exoenzyme, or the Rho kinase inhibitor, Y-27632, had no effect. In TNFα-treated HAECs, pravastatin inhibited Rac1 and Cdc42 activation and their translocation to the cell membrane. Blocking the transcriptional activation of NF-κB prevented the TNFα-induced downregulation of TM. The pravastatin-induced increase in TM expression in TNFα-treated HAECs was mediated through inhibition of NF-κB activation. Pravastatin regulates TM expression by inhibiting the activation of the Rho family proteins, Rac1 and Cdc42, and the transcription factor, NF-κB. The increase in endothelial TM activity in response to pravastatin constitutes a novel pleiotropic (nonlipid-related) effect of this commonly used compound and may be of clinical significance in disorders in which deficient endothelial TM plays a pathophysiological role.

原文英語
頁(從 - 到)642-653
頁數12
期刊Journal of Cellular Biochemistry
101
發行號3
DOIs
出版狀態已發佈 - 六月 1 2007
對外發佈Yes

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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