Pparα deficiency inhibits the proliferation of neuronal and glial precursors in the zebrafish central nervous system

Yen Che Hsieh, Ming Chang Chiang, Yin Cheng Huang, Tu Hsueh Yeh, Hung Yu Shih, Han Fang Liu, Hao Yuan Chen, Chien Ping Wang, Yi Chuan Cheng

研究成果: 雜誌貢獻文章

摘要

Background: Many molecules and signaling pathways involved in neural development play a role in neurodegenerative diseases and brain tumor progression. Peroxisome proliferator-activated receptor (PPAR) proteins regulate the differentiation of tissues and the progression of many diseases. However, the role of these proteins in neural development is unclear. Results: We examined the function of Pparα in the neural development of zebrafish. Two duplicate paralogs for mammalian PPARA/Ppara, namely pparaa and pparab, are present in the zebrafish genome. Both pparaa and pparab are expressed in the developing central nervous system in zebrafish embryos. Inhibiting the function of Pparα by using either the PPARα/Pparα antagonist GW6471 or pparaa or pparab truncated constructs produced identical phenotypes, which were sufficient to reduce the proliferation of neuronal and glial precursor cells without affecting the formation of neural progenitors. Conclusions: We demonstrated that both Pparαa and Pparαb proteins are essential regulators of the proliferation of neuronal and glial precursors. This study provides a better understanding of the functions of PPARα/Pparα in neural development and further expands our knowledge of the potential role of PPARα/Pparα in neurological disorders and brain tumors. Developmental Dynamics 247:1264–1275, 2018. © 2018 Wiley Periodicals, Inc.
原文英語
頁(從 - 到)1264-1275
頁數12
期刊Developmental Dynamics
247
發行號12
DOIs
出版狀態已發佈 - 十二月 1 2018

指紋

Peroxisome Proliferator-Activated Receptors
Zebrafish
Neuroglia
Central Nervous System
Brain Neoplasms
Proteins
Nervous System Diseases
Neurodegenerative Diseases
Disease Progression
Embryonic Structures
Genome
Phenotype

Keywords

  • glial precursor
  • neural development
  • neuronal precursor
  • Pparα
  • zebrafish

ASJC Scopus subject areas

  • Developmental Biology

引用此文

Pparα deficiency inhibits the proliferation of neuronal and glial precursors in the zebrafish central nervous system. / Hsieh, Yen Che; Chiang, Ming Chang; Huang, Yin Cheng; Yeh, Tu Hsueh; Shih, Hung Yu; Liu, Han Fang; Chen, Hao Yuan; Wang, Chien Ping; Cheng, Yi Chuan.

於: Developmental Dynamics, 卷 247, 編號 12, 01.12.2018, p. 1264-1275.

研究成果: 雜誌貢獻文章

Hsieh, YC, Chiang, MC, Huang, YC, Yeh, TH, Shih, HY, Liu, HF, Chen, HY, Wang, CP & Cheng, YC 2018, 'Pparα deficiency inhibits the proliferation of neuronal and glial precursors in the zebrafish central nervous system', Developmental Dynamics, 卷 247, 編號 12, 頁 1264-1275. https://doi.org/10.1002/dvdy.24683
Hsieh, Yen Che ; Chiang, Ming Chang ; Huang, Yin Cheng ; Yeh, Tu Hsueh ; Shih, Hung Yu ; Liu, Han Fang ; Chen, Hao Yuan ; Wang, Chien Ping ; Cheng, Yi Chuan. / Pparα deficiency inhibits the proliferation of neuronal and glial precursors in the zebrafish central nervous system. 於: Developmental Dynamics. 2018 ; 卷 247, 編號 12. 頁 1264-1275.
@article{8a58862b056e44efa0a399a62761202a,
title = "Pparα deficiency inhibits the proliferation of neuronal and glial precursors in the zebrafish central nervous system",
abstract = "Background: Many molecules and signaling pathways involved in neural development play a role in neurodegenerative diseases and brain tumor progression. Peroxisome proliferator-activated receptor (PPAR) proteins regulate the differentiation of tissues and the progression of many diseases. However, the role of these proteins in neural development is unclear. Results: We examined the function of Pparα in the neural development of zebrafish. Two duplicate paralogs for mammalian PPARA/Ppara, namely pparaa and pparab, are present in the zebrafish genome. Both pparaa and pparab are expressed in the developing central nervous system in zebrafish embryos. Inhibiting the function of Pparα by using either the PPARα/Pparα antagonist GW6471 or pparaa or pparab truncated constructs produced identical phenotypes, which were sufficient to reduce the proliferation of neuronal and glial precursor cells without affecting the formation of neural progenitors. Conclusions: We demonstrated that both Pparαa and Pparαb proteins are essential regulators of the proliferation of neuronal and glial precursors. This study provides a better understanding of the functions of PPARα/Pparα in neural development and further expands our knowledge of the potential role of PPARα/Pparα in neurological disorders and brain tumors. Developmental Dynamics 247:1264–1275, 2018.",
keywords = "glial precursor, neural development, neuronal precursor, Pparα, zebrafish, glial precursor, neural development, neuronal precursor, Pparα, zebrafish",
author = "Hsieh, {Yen Che} and Chiang, {Ming Chang} and Huang, {Yin Cheng} and Yeh, {Tu Hsueh} and Shih, {Hung Yu} and Liu, {Han Fang} and Chen, {Hao Yuan} and Wang, {Chien Ping} and Cheng, {Yi Chuan}",
year = "2018",
month = "12",
day = "1",
doi = "10.1002/dvdy.24683",
language = "English",
volume = "247",
pages = "1264--1275",
journal = "Developmental Dynamics",
issn = "1058-8388",
publisher = "Wiley-Liss Inc.",
number = "12",

}

TY - JOUR

T1 - Pparα deficiency inhibits the proliferation of neuronal and glial precursors in the zebrafish central nervous system

AU - Hsieh, Yen Che

AU - Chiang, Ming Chang

AU - Huang, Yin Cheng

AU - Yeh, Tu Hsueh

AU - Shih, Hung Yu

AU - Liu, Han Fang

AU - Chen, Hao Yuan

AU - Wang, Chien Ping

AU - Cheng, Yi Chuan

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Background: Many molecules and signaling pathways involved in neural development play a role in neurodegenerative diseases and brain tumor progression. Peroxisome proliferator-activated receptor (PPAR) proteins regulate the differentiation of tissues and the progression of many diseases. However, the role of these proteins in neural development is unclear. Results: We examined the function of Pparα in the neural development of zebrafish. Two duplicate paralogs for mammalian PPARA/Ppara, namely pparaa and pparab, are present in the zebrafish genome. Both pparaa and pparab are expressed in the developing central nervous system in zebrafish embryos. Inhibiting the function of Pparα by using either the PPARα/Pparα antagonist GW6471 or pparaa or pparab truncated constructs produced identical phenotypes, which were sufficient to reduce the proliferation of neuronal and glial precursor cells without affecting the formation of neural progenitors. Conclusions: We demonstrated that both Pparαa and Pparαb proteins are essential regulators of the proliferation of neuronal and glial precursors. This study provides a better understanding of the functions of PPARα/Pparα in neural development and further expands our knowledge of the potential role of PPARα/Pparα in neurological disorders and brain tumors. Developmental Dynamics 247:1264–1275, 2018.

AB - Background: Many molecules and signaling pathways involved in neural development play a role in neurodegenerative diseases and brain tumor progression. Peroxisome proliferator-activated receptor (PPAR) proteins regulate the differentiation of tissues and the progression of many diseases. However, the role of these proteins in neural development is unclear. Results: We examined the function of Pparα in the neural development of zebrafish. Two duplicate paralogs for mammalian PPARA/Ppara, namely pparaa and pparab, are present in the zebrafish genome. Both pparaa and pparab are expressed in the developing central nervous system in zebrafish embryos. Inhibiting the function of Pparα by using either the PPARα/Pparα antagonist GW6471 or pparaa or pparab truncated constructs produced identical phenotypes, which were sufficient to reduce the proliferation of neuronal and glial precursor cells without affecting the formation of neural progenitors. Conclusions: We demonstrated that both Pparαa and Pparαb proteins are essential regulators of the proliferation of neuronal and glial precursors. This study provides a better understanding of the functions of PPARα/Pparα in neural development and further expands our knowledge of the potential role of PPARα/Pparα in neurological disorders and brain tumors. Developmental Dynamics 247:1264–1275, 2018.

KW - glial precursor

KW - neural development

KW - neuronal precursor

KW - Pparα

KW - zebrafish

KW - glial precursor

KW - neural development

KW - neuronal precursor

KW - Pparα

KW - zebrafish

UR - http://www.scopus.com/inward/record.url?scp=85057331050&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85057331050&partnerID=8YFLogxK

U2 - 10.1002/dvdy.24683

DO - 10.1002/dvdy.24683

M3 - Article

C2 - 30358936

AN - SCOPUS:85057331050

VL - 247

SP - 1264

EP - 1275

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 12

ER -