The effects of the src gene on the activity of protein kinase C and intercellular communication have been studied in transformed NIH/3T3 clones isolated from soft agar following transfection with the plasmid carrying the v-src gene (p(src-11)). Six transformed clones that were studied contained newly incorporated v-src genes in the genome, had an increased amount of pp60(src), and showed enhanced activities of protein kinase C. Intercellular communication, studied by observing with autoradiography the transfer of [3H]uridine nucleotide from prelabeled donor cells to recipient cells in contact, was found to be reduced in transformed clones as compared to parental NIH/3T3 cells. Treatment with phorbol 12-myristate 13-acetate was also found to increase protein kinase C activity and to reduce intercellular communication in normal NIH/3T3 cells. These results suggest that the v-src gene product, in a manner similar to some of the powerful tumor promoters, may directly or indirectly affect cell-cell communication.
|頁（從 - 到）||5360-5364|
|期刊||Proceedings of the National Academy of Sciences of the United States of America|
|出版狀態||已發佈 - 十一月 6 1985|
ASJC Scopus subject areas
Chang, C. C., Trosko, J. E., Kung, H. J., Bombick, D., & Matsumura, F. (1985). Potential role of the src gene product in inhibition of gap-junctional communication in NIH/3T3 cells. Proceedings of the National Academy of Sciences of the United States of America, 82(16), 5360-5364. https://doi.org/10.1073/pnas.82.16.5360