Potential role of the src gene product in inhibition of gap-junctional communication in NIH/3T3 cells

C. C. Chang, J. E. Trosko, H. J. Kung, D. Bombick, F. Matsumura

研究成果: 雜誌貢獻文章

84 引文 斯高帕斯(Scopus)

摘要

The effects of the src gene on the activity of protein kinase C and intercellular communication have been studied in transformed NIH/3T3 clones isolated from soft agar following transfection with the plasmid carrying the v-src gene (p(src-11)). Six transformed clones that were studied contained newly incorporated v-src genes in the genome, had an increased amount of pp60(src), and showed enhanced activities of protein kinase C. Intercellular communication, studied by observing with autoradiography the transfer of [3H]uridine nucleotide from prelabeled donor cells to recipient cells in contact, was found to be reduced in transformed clones as compared to parental NIH/3T3 cells. Treatment with phorbol 12-myristate 13-acetate was also found to increase protein kinase C activity and to reduce intercellular communication in normal NIH/3T3 cells. These results suggest that the v-src gene product, in a manner similar to some of the powerful tumor promoters, may directly or indirectly affect cell-cell communication.
原文英語
頁(從 - 到)5360-5364
頁數5
期刊Proceedings of the National Academy of Sciences of the United States of America
82
發行號16
DOIs
出版狀態已發佈 - 十一月 6 1985
對外發佈Yes

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