Post conductive keratoplasty visualization of rabbit cornea by multiphoton microscopy

Wen Lo, Tsung Jen Wang, Fung Rong Hu, Chen Yuan Dong

研究成果: 書貢獻/報告類型會議貢獻

摘要

Conductive keratoplasty (CK) is a new refractive surgery for presbyopia and hyperopia patients. By applying radio frequency current at the peripheral regions of cornea, collagen, the most abundant composition of corneal stroma, shrinks due to the heat generated. The shrinkage at the periphery alters the corneal architecture and achieves clearer focus for near vision. In this work we use multiphoton microscopy to observe the post surgery structure variation at both submicron resolution and over a large region within the tissue. Since collagen can be induced to generate strong second harmonic generation (SHG) signal, multiphoton excitation provide direct visualization of collagen orientation within corneal stroma. In addition, since the SHG intensity of collagen tissue deteriorates with increasing thermal damage [1-3], our methodology can be used to characterize the extent of corneal stroma damage from the CK procedure. Finally, the influence of CK on the morphology and distribution of keratocytes can also be investigated by detecting multiphoton excited autofluorescence from the cells.
原文英語
主出版物標題Confocal, Multiphoton, and Nonlinear Microscopic Imaging III
出版狀態已發佈 - 十一月 26 2007
事件Confocal, Multiphoton, and Nonlinear Microscopic Imaging III - Munich, 德国
持續時間: 六月 19 2007六月 21 2007

出版系列

名字Progress in Biomedical Optics and Imaging - Proceedings of SPIE
6630
ISSN(列印)1605-7422

會議

會議Confocal, Multiphoton, and Nonlinear Microscopic Imaging III
國家德国
城市Munich
期間6/19/076/21/07

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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    Lo, W., Wang, T. J., Hu, F. R., & Dong, C. Y. (2007). Post conductive keratoplasty visualization of rabbit cornea by multiphoton microscopy. 於 Confocal, Multiphoton, and Nonlinear Microscopic Imaging III [663006] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; 卷 6630).