Positional candidate gene approach and functional genomics strategy in atopy gene discovery

R. G. Nickel, F. P. Saitta, L. R. Freidhoff, X. Y. Yu, E. Ehrlich, K. C. Barnes, T. Beaty, Shau Ku Huang

研究成果: 雜誌貢獻文章

7 引文 (Scopus)

摘要

As part of our effort in searching for genetic factors contributing to the susceptibility to atopy and asthma, we have focused on a 'positional candidate' approach in identifying CC chemokine gene polymorphisms and their functional correlates. To date, a single-nucleotide polymorphism was found in the RANTES proximal promoter region, and a high degree of sequence variation was identified in the 3'-untranslated region -of the eotaxin gene. Also, we are pursuing a series of functional genomics' studies designed to identify differentially expressed genes in a panel of allergen-specific human Th2 cells and in antigen-induced hyperreactive murine airways. This is performed using a combination of protocols including suppression-subtractive hybridization and cDNA array hybridizations with 18,363 nonredundant sequences. A data base is being generated from a list of subtracted cDNA sequences and array-positive clones to categorize differentially expressed genes. Sequences are being placed in biologically relevant categories on the basis of function (i.e., receptor, signal transduction pathways, transcription, and translation). With the increasing amount of sequence information compiled by the Human Genome Project, it will be particularly challenging to integrate functional gene-mapping efforts to define and compare aberrant genotypes/phenotypes in atopic diseases.

原文英語
頁(從 - 到)282-284
頁數3
期刊International Archives of Allergy and Immunology
118
發行號2-4
DOIs
出版狀態已發佈 - 一月 1 1999
對外發佈Yes

指紋

Genetic Association Studies
Genomics
Oligonucleotide Array Sequence Analysis
Genes
Human Genome Project
Chemokine CCL5
CC Chemokines
Th2 Cells
Chromosome Mapping
3' Untranslated Regions
Genetic Promoter Regions
Allergens
Single Nucleotide Polymorphism
Signal Transduction
Asthma
Clone Cells
Genotype
Databases
Phenotype
Antigens

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

引用此文

Positional candidate gene approach and functional genomics strategy in atopy gene discovery. / Nickel, R. G.; Saitta, F. P.; Freidhoff, L. R.; Yu, X. Y.; Ehrlich, E.; Barnes, K. C.; Beaty, T.; Huang, Shau Ku.

於: International Archives of Allergy and Immunology, 卷 118, 編號 2-4, 01.01.1999, p. 282-284.

研究成果: 雜誌貢獻文章

Nickel, RG, Saitta, FP, Freidhoff, LR, Yu, XY, Ehrlich, E, Barnes, KC, Beaty, T & Huang, SK 1999, 'Positional candidate gene approach and functional genomics strategy in atopy gene discovery', International Archives of Allergy and Immunology, 卷 118, 編號 2-4, 頁 282-284. https://doi.org/10.1159/000024100
Nickel, R. G. ; Saitta, F. P. ; Freidhoff, L. R. ; Yu, X. Y. ; Ehrlich, E. ; Barnes, K. C. ; Beaty, T. ; Huang, Shau Ku. / Positional candidate gene approach and functional genomics strategy in atopy gene discovery. 於: International Archives of Allergy and Immunology. 1999 ; 卷 118, 編號 2-4. 頁 282-284.
@article{a4b639e88a9b4008b149632c37d62ad3,
title = "Positional candidate gene approach and functional genomics strategy in atopy gene discovery",
abstract = "As part of our effort in searching for genetic factors contributing to the susceptibility to atopy and asthma, we have focused on a 'positional candidate' approach in identifying CC chemokine gene polymorphisms and their functional correlates. To date, a single-nucleotide polymorphism was found in the RANTES proximal promoter region, and a high degree of sequence variation was identified in the 3'-untranslated region -of the eotaxin gene. Also, we are pursuing a series of functional genomics' studies designed to identify differentially expressed genes in a panel of allergen-specific human Th2 cells and in antigen-induced hyperreactive murine airways. This is performed using a combination of protocols including suppression-subtractive hybridization and cDNA array hybridizations with 18,363 nonredundant sequences. A data base is being generated from a list of subtracted cDNA sequences and array-positive clones to categorize differentially expressed genes. Sequences are being placed in biologically relevant categories on the basis of function (i.e., receptor, signal transduction pathways, transcription, and translation). With the increasing amount of sequence information compiled by the Human Genome Project, it will be particularly challenging to integrate functional gene-mapping efforts to define and compare aberrant genotypes/phenotypes in atopic diseases.",
keywords = "Asthma, Atopy, Candidate gene approach, CC chemokine, cDNA/oligonucleotide array, Functional genomics",
author = "Nickel, {R. G.} and Saitta, {F. P.} and Freidhoff, {L. R.} and Yu, {X. Y.} and E. Ehrlich and Barnes, {K. C.} and T. Beaty and Huang, {Shau Ku}",
year = "1999",
month = "1",
day = "1",
doi = "10.1159/000024100",
language = "English",
volume = "118",
pages = "282--284",
journal = "International Archives of Allergy and Immunology",
issn = "1018-2438",
publisher = "S. Karger AG",
number = "2-4",

}

TY - JOUR

T1 - Positional candidate gene approach and functional genomics strategy in atopy gene discovery

AU - Nickel, R. G.

AU - Saitta, F. P.

AU - Freidhoff, L. R.

AU - Yu, X. Y.

AU - Ehrlich, E.

AU - Barnes, K. C.

AU - Beaty, T.

AU - Huang, Shau Ku

PY - 1999/1/1

Y1 - 1999/1/1

N2 - As part of our effort in searching for genetic factors contributing to the susceptibility to atopy and asthma, we have focused on a 'positional candidate' approach in identifying CC chemokine gene polymorphisms and their functional correlates. To date, a single-nucleotide polymorphism was found in the RANTES proximal promoter region, and a high degree of sequence variation was identified in the 3'-untranslated region -of the eotaxin gene. Also, we are pursuing a series of functional genomics' studies designed to identify differentially expressed genes in a panel of allergen-specific human Th2 cells and in antigen-induced hyperreactive murine airways. This is performed using a combination of protocols including suppression-subtractive hybridization and cDNA array hybridizations with 18,363 nonredundant sequences. A data base is being generated from a list of subtracted cDNA sequences and array-positive clones to categorize differentially expressed genes. Sequences are being placed in biologically relevant categories on the basis of function (i.e., receptor, signal transduction pathways, transcription, and translation). With the increasing amount of sequence information compiled by the Human Genome Project, it will be particularly challenging to integrate functional gene-mapping efforts to define and compare aberrant genotypes/phenotypes in atopic diseases.

AB - As part of our effort in searching for genetic factors contributing to the susceptibility to atopy and asthma, we have focused on a 'positional candidate' approach in identifying CC chemokine gene polymorphisms and their functional correlates. To date, a single-nucleotide polymorphism was found in the RANTES proximal promoter region, and a high degree of sequence variation was identified in the 3'-untranslated region -of the eotaxin gene. Also, we are pursuing a series of functional genomics' studies designed to identify differentially expressed genes in a panel of allergen-specific human Th2 cells and in antigen-induced hyperreactive murine airways. This is performed using a combination of protocols including suppression-subtractive hybridization and cDNA array hybridizations with 18,363 nonredundant sequences. A data base is being generated from a list of subtracted cDNA sequences and array-positive clones to categorize differentially expressed genes. Sequences are being placed in biologically relevant categories on the basis of function (i.e., receptor, signal transduction pathways, transcription, and translation). With the increasing amount of sequence information compiled by the Human Genome Project, it will be particularly challenging to integrate functional gene-mapping efforts to define and compare aberrant genotypes/phenotypes in atopic diseases.

KW - Asthma

KW - Atopy

KW - Candidate gene approach

KW - CC chemokine

KW - cDNA/oligonucleotide array

KW - Functional genomics

UR - http://www.scopus.com/inward/record.url?scp=0032915982&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032915982&partnerID=8YFLogxK

U2 - 10.1159/000024100

DO - 10.1159/000024100

M3 - Article

C2 - 10224411

AN - SCOPUS:0032915982

VL - 118

SP - 282

EP - 284

JO - International Archives of Allergy and Immunology

JF - International Archives of Allergy and Immunology

SN - 1018-2438

IS - 2-4

ER -